Centre National De La Recherche Scientifique

Université Pierre Et Marie Curie (Paris 6)

Université Pierre Et Marie Curie

UniversitÉ De Versailles—saint-Quentin-En-Yvelines

Ecole Pratique Des Hautes Etudes

Sorbonne Université

Centre National De La Recherche Scientifique-cnrs

Nadine Nassif

Clément Sanchez

Marie-Madeleine Giraud-Guille

Clement Sanchez

Cedric Boissiere

Cédric Boissiere

Gervaise Mosser

Frédéric Gobeaux

Yan Wang

Christophe Helary

Anne Pelle

Marie-Madeleine Giraud Guille

Francisco Miguel Moreira Martins Fernandes

Frederic Gobeaux

Marie Madeleine Giraud Guille

Disclosed is the preparation of injectable collagen suspensions, to the method for preparing the suspensions, and to the uses thereof, particularly for forming dense collagen matrices.

Injectable collagen suspensions, the preparation method thereof, and the uses thereof, particularly for forming dense collagen matrices

A method for preparing a synthetic material having an organic phase and a mineral phase which consists in preparing an initial acidic aqueous solution of collagen which is a precursor for the organic phase (I), and at least one aqueous solution of precursors for the mineral phase (II), and in precipitating the collagen by increasing the pH to a value of at least 7. The method includes the concentration of collagen in the acidic aqueous solution is at least 75 mg/ml and remains constant during said increase in pH. The mineral phase precursors have at least one calcium salt and at least one phosphate salt. The precipitation of the mineral phase (II) is carried out by bringing the mineral phase precursor solution into contact with the organic phase (I), the bringing into contact being carried out before or after the precipitation of the organic phase (I).

Bone substitute, and method for the preparation thereof

Method for preparing a homogeneous collagen-based material by concentration of a collagen solution, includes bringing a collagen solution into contact by way of continuous injection and use of the material for tissue repair.

Dense fibrillar collagen matrices for tissue repair and the preparation method thereof

The invention relates to a material that can be used as a bone substitute and to a method for the preparation thereof. The material comprises an organic phase (I) comprising striated collagen fibrils constituted of collagen I triple helices, said fibrils being organized over a large distance according to a 3D geometry associating aligned domains and cholesteric domains, and also isotropic domains where they are not organized; and a mineral phase (II) comprising apatite crystals having a hexagonal crystalline structure, space group 6/m, comprising at least calcium ions and phosphate ions; the axis c of said apatite crystals of the mineral phase is coaligned with the longitudinal axis of the striated collagen fibrils of the organic phase and the collagen content in the material is at least 75 mg/cm. The method for preparing the material comprises preparing an initial acidic aqueous solution of collagen which is a precursor for the organic phase (I), and at least one aqueous solution of precursors for the mineral phase (II) and precipitating the collagen by increasing the pH to a value of at least 7. According to this method: the concentration of collagen in the acidic aqueous solution is at least 75 mg/ml and remains constant during said increase in pH; the mineral phase precursors comprise at least one calcium salt and at least one phosphate salt; the precipitation of the mineral phase (II) is carried out by bringing the mineral phase precursor solution into contact with the organic phase (I), said bringing into contact being carried out before or after the precipitation of said organic phase (I).

Bone substitute and method for the preparation thereof

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Paris, France

Nadine Nassif