Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Commissariat a L'energie Atomique

Nadine Coulon

Nicolas Ugolin

Sylvie Chevillard

Julien Le Meur

Denis Menut

Caroline Falck

Jean-Marc Joseph Desaulniers

Sophie Giraud

Emilie Lefevre

Emilie Bosc

A process, for the quantitative analysis by optical emission spectroscopy of a plasma induced by a laser beam of at least one target included in a biochip, involves the use of an adjuvant allowing the formation of a dry matrix capable of being ablated simultaneously with the at least one target, the dry matrix being configured to improve the analytical properties of plasma, the adjuvant having an emission spectrum whose lines have wavelengths distinct from the wavelengths of the spectral lines used for the quantitative analysis of said at least one target.

Method and device for the quantitative LIBS measurement of bio-molecular targets on a biochip

The present invention relates to a device and method for the high speed quantitative measurement of biomolecular targets on the surface or in the body of a planar medium for biological analysis. The method, according to the invention, includes the following, steps: a) at least two laser beams (F&#x2033;) are focused and overlaid on each measuring point of said medium by the simultaneous intersection of these beams to extract a contained hot plasma (P), including a measured chemical element present in the targets and another chemical element exogenous to the targets and present in a known quantity on this medium; b) luminous emission rays for each plasma, corresponding to the quantified element and exogenous element, are detected and analyzed for each measuring point while measuring the brightness of these rays; then c) the concentration in each measuring point of the quantified element is determined through prior calibration of the rays of the qualified element to determine a correlation between the brightness of the rays, specific to said element, and the concentrations of the latter in mixtures of the quantified element and exogenous element in known proportions.

Method and device for high speed quantitative measurement of biomolecular targets on or in biological analysis medium

The invention relates to a method for manufacturing an assembly () for a fuel cell comprising at least two adjacent cell components, applied by means of the successive steps consisting of: a) solidifying by laser sintering a powder layer (Ci) deposited beforehand so that it forms a section of the assembly; and b) depositing a successive powder layer on the powder layer (Ci) deposited beforehand and solidified by laser sintering; the steps a) and b) being alternately repeated until the obtained stacked sections form together the assembly comprising at least two components. According to the invention, the method is applied so that at least one of the obtained sections (S-Sp) has at least two areas with different porosities.

Method for manufacturing an assembly for a fuel cell

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Dourdan, France

Nadine Coulon