Myriad Genetics, Inc.

Nadeem Tusneem

Min-Jui Richard Shen

Dmitry Pruss

Satish K Bhatnagar

A method for normalizing the intensity of G bands in sequencing methods which utilize dITP is presented. The use of dITP normally results in decreased intensities of G bands which occur after A bands, i.e., in the sequence AG. It has been discovered that the use of ddITP in place of ddGTP or in conjunction with ddGTP helps to normalize the intensity of the G bands following A bands. This aids in preventing errors in reading sequencing chromatograms and allows for extended reads of sequencing chromatograms as compared to methods which utilize dITP without the use of ddITP.

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West Jordan, UT, United States of America

Nadeem Tusneem