Scios Inc.

University of Medicine and Dentistry of New Jersey

N Stephen Pollitt

Masayori Inouye

Judith A Abraham

Weining Jiang

Joel E Goldstein

Jean-Pierre Etchegaray

Pamela Jones

Ziyang Zhong

Douglas I Buckley

Taymar E Hartman

Peter A Stathis

The invention is directed to a method of enhancing the biological activity of vascular endothelial growth factors (VEGF). The invention further concerns certain VEGF variants having enhanced biological activity, methods and means for preparing these variants, and pharmaceutical compositions comprising them. In a further aspect, the invention concerns methods of treatment using, and articles of manufacture containing such VEGF variants.

Vascular endothelial growth factor variants

A family of stimuli-induced in particular stress or cold-shock induced genes and proteins are disclosed which have conserved amino acid domains. Nucleic acid sequences of the genes and the promoters are also described. Various utilities of the promoters and of the proteins are disclosed.

Nucleic acids sequence, stress-induced proteins and uses thereof

A method is described for producing a peptide having a pI above 8 or below 5 wherein the peptide is expressed as a fusion protein in which it is linked to a fusion partner through an acid cleavage site. The peptide is released from the fusion partner by acid cleavage in the absence of chaotrope. The fusion partner and its acid cleavage products, if any, have a net charge sufficiently different from that of the desired peptide to allow isolation of the peptide by ion-exchange chromatography.

Method for producing a peptide with a pI above 8 or below 5

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Los Altos, CA, United States of America

N Stephen Pollitt