Sloan-kettering Institute for Cancer Research

New York Medical College

Sloan-kettering Institute

Myron R Melamed

Lloyd J Old

Herbert F Oettgen

Carlos Cordon-Cardo

Kenneth O Lloyd

Wolfgang J Rettig

Connie L Finstad

Pilar Garin-Chesa

Junichi Sakamoto

Willet F Whitmore

Neil H Bander

Bernd R Anger

Eileen A Friedman

H Richard Beresford

Willet F Whitemore, Jr

Yves Fradet

Hans-Joachim Feickert

Karen Chorney

Frank N Traganos

Xun Li

Zbigniew D Darzynkiewicz

Warren E Enker

A method for detecting halogenated precursors incorporated into DNA is presented. The method is based on the selective photolysis of DNA by ultraviolet (UV) light at the sites of an incorporated halogenated precursor, such as the thymidine base analogs 5-bromo-2-deoxyuridine (BrdUrd), 5-iodo-2-deoxyuridine (IdUrd), 5-fluoro-2-deoxyuridine (FdUrd), or 5-chloro-2-deoxyuridine (CldUrd). The 3'-hydroxyl termini of the DNA single strand breaks generated during photolysis may be marked directly or indirectly with a fluorescent label. The DNA termini are directly labeled with fluorochrome-conjugated deoxyuridine triphosphate (dUTP) catalyzed by exogenous terminal deoxynucleotidyl transferase or DNA polymerase (nick translation system). The DNA termini are indirectly labeled with either biotin- or digoxygenin-conjugated dUTP; the incorporated biotin or digoxygenin is then detected following binding of fluorochrome-conjugated avidin or anti-digoxygenin antibody, respectively. The labeled DNA may be analyzed in situ by flow cytometry or fluorescence microscopy. Alternatively, the DNA may be isolated and analyzed by conventional methods, including gel electrophoresis and blotting assays, prior to marking with a flourescent label. The method does not require denaturation of the DNA and may be used with cells in suspension, thin tissue sections, bacteria, and viruses. The method has application in the analysis of cell proliferation and genotoxicity tests.

Detection of halogenated precursors incorporated into DNA

This invention provides a method of identifying mesenchymal tissues as normal, proliferatively active or malignant. This invention also provides a method of distinguishing subsets of sarcomas with distinctive antigenic phenotypes. This invention also provides a method of diagnosing mesenchymal tumors. Finally, this invention provides a monoclonal antibody designated G171 and the hybridoma cell line producing said monoclonal antibody (ATCC No. HB9254).

Cell-surface glycoproteins of human sarcomas

Antigenic profiles of renal carcinoma specimans developed with panels of monoclonal antibodies derived from several different tissues serve as useful clinical indicators for cancer type, cancer subset as well as histiogenesis and prognosis indicators.

Method for characterizing types of renal carcinoma and prognosis

A panel of monoclonal antibodies produced from normal human lung fibroblasts and human lung tumors as immunogen is used to diagnose the presence of lung tumors and differentiate between those which are benign and those which are cancerous.

Monoclonal antibodies to human lung cancers and method

A panel of monoclonal antibodies, produced from human bladder tumors as immunogen, is used to diagnose the presence of transitional cell carcinoma in patients. The panel is also used to identify and differentiate low grade non-invasive papillomas from invasive life-threatening transitional cell carcinomas, thereby enabling decisions as to the extent of bladder surgery. These mAbs can also be used as a panel for tissue typing of normal and abnormal cell specimens.

Monoclonal antibodies to human bladder and ureter cancers and method

A panel of monoclonal antibodies produced from human gastrointestinal tumors as immunogen is used to diagnose the presence of colon cancer. The antibody panel subsets the human gastrointestinal tract in its reactivity vis-a-vis esophagus, stomach, small intestine and colon. The panel is useful as a diagnostic probe for cancer.

Monoclonal antibodies to human gastrointestinal cancers and hybridoma

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Dobbs Ferry, NY, United States of America

Myron R Melamed