Abbott Laboratories Corporation

Myrna G Ulanday

Mark W Bailey

A reagent system for determing the hemoglobin content of whole blood comprising an aqueous solution of polyvinylpyrrolidone of molecular weight from about 10,000 to about 360,000 at a pH greater than about 8.0 to denature hemoglobin in whole blood. A method is also provided wherein a blood sample is treted with an aqueous solution of polyvinylpyrrolidone of molecular weight from about 10,000 to about 360,000 at a basic pH sufficient to denature the hemoglobin present in the whole blood to form a stable product whereafter the absorbance of the stable product of the blood sample and polyvinylpyrrolidone of molecular weight from about 10,000 to about 360,000 polymer reagent system is measured at a wavelength of approximately 575 nm to determine the hemoglobin contentration.

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Libertyville, IL, United States of America

Myrna G Ulanday