Genomatica, Inc.

Reg Life Sciences, LLC

Myong K Ko

Haibo Wang

Perry Y Liao

Simon Li

Andreas W Schirmer

Stephen B Del Cardayre

Fernando A Sanchez-Riera

Lauri H Suominen

Patricia J Cole

Jefferson Lievense

Jorge Jesus Martinez-Gonzalez

Aiguo Liu

Wei Nan

The present disclosure relates to chemo-enzymatic processes for the preparation of lactones (including, e.g., macrolactones, γ-lactones, and δ-lactones) and/or macrocyclic ketones, which are compounds of industrial value, for example, for use as fragrance ingredients. The chemo-enzymatic processes combine the in vivo microbial production of fatty acid derivatives and the subsequent ex vivo synthetic transformation of the fatty acid derivatives to provide the lactones and macrocyclic ketones.

Semisynthetic routes to organic compounds

Methods of recovering cannabinoids from cell cultures include methods comprising steps of separating the cell culture at a temperature above the melting point of the cannabinoid to separate a light phase comprising liquid state cannabinoid from a heavy phase; and methods comprising treating the cell culture at a temperature below the melting point of the cannabinoid to separate a light phase from a heavy phase comprising solid state cannabinoid. Other methods include contacting the culture with a water-miscible solvent to form a water-miscible phase and an aqueous phase, separating the two phases and recovering the cannabinoid. Other methods include contacting the culture with a water-immiscible solvent to form a water-immiscible phase and an aqueous phase, separating the two phases, and recovering the cannabinoid. Other methods include washing the inner surface of a fermentation vessel with alkaline solution to recover cannabinoid attached to the vessel surface. Various methods make use of aqueous solvent systems comprising no organic solvent, aqueous solvent systems comprising added water-miscible organic solvent, and dual-phase aqueous/water-immiscible solvent systems.

Recovery, decarboxylation, and purification of cannabinoids from engineered cell cultures

The disclosure relates to downstream processing of fatty alcohol (FALC) and provides a novel purification method that provides FALC at high purity and yield.

Downstream processing of fatty alcohol compositions produced by recombinant host cells

The present disclosure relates to processes that combine microbial production of organic intermediates and subsequent synthetic transformation to provide compounds of industrial value, including compounds used in fragrances.

Chemo-enzymatic process

The disclosure relates to a novel glycerol purification process that produces partially refined waste glycerol for a variety of industrial applications. The disclosure encompasses a salt-containing partially refined glycerol composition that is suitable as a fermentation grade glycerol.

Production of partially refined waste glycerol

Growing community of inventors

South San Francisco, CA, United States of America

Myong K Ko