Dsm IP Assets B.v.

Ge Healthcare Bio-sciences Ab

Mylene Caussette

Inger Lagerlund

Johan Fährenmark

Robert Morenweiser

Eric Piron

Frederik Miggelbrink

Gregory Maufroid

De André Haan

We describe a method for purifying chymosin comprising providing an aqueous liquid sample containing chymosin and a separation medium comprising a base matrix and a plurality of firmly attached ligands that are capable of binding to chymosin, contacting the matrix with the sample under conditions permitting binding of chymosin to the matrix, and desorbing chymosin from the matrix. The characterizing feature is that the matrix is hydrophilic and that the ligands in the plurality of ligands are hydrocarbon groups in which all carbon atoms are sp-hybridised, possibly with an ether oxygen or a thioether sulphur inserted between two carbon atoms at one or more positions in at least one of the hydrocarbon groups, and possibly a hydroxy group replacing a hydrogen atom at one or more positions in at least one of the hydrocarbon groups.

Method for the purification of chymosin

The invention relates to a method for the inactivation of amylase in a solution comprisingaspartic protease by keeping the solution at a pH between 2.1 and 2.8 and at a temperature between 20&#xb0; C. and 40&#xb0; C. for a period of time sufficient to inactivate amylase for at least 95%. With this method the level of residualaspartic protease activity in the solution at the end of the heating time is at least 90%, preferably at least 95%, and the residual amylase activity is 0.05 RAU/g or lower, more preferably 0.001 RAU/g or lower, i.e. more than 99% of the amylase activity has been inactivated.

Method for the inactivation of amylase in the presence of protease

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Lille, France

Mylene Caussette