Monsanto Technology LLC

Monsanto Company

Sangamo Biosciences, Inc.

Mylavarapu Venkatramesh

Ganesh M Kishore

Balasulojini Karunanandaa

John R LeDeaux

Martha Post-Beittenmiller

Gregory M Thorne

Henry E Valentin

Tyamagondlu V Venkatesh

Sekhar S Boddupalli

Albert Boronat

Narciso Campos

Shaukat H Rangwala

Manual Rodriguez-Concepcion

Michel Rohmer

Myriam Seemann

David Richard Corbin

Edward J Rebar

Michael W Lassner

Andrew Jamieson

Jingdong Liu

Qiang Liu

Kathryn Dennis Lardizabal

Guofu Li

B Ganesh Bhat

Barkur G Bhat

Robert J Grebenok

John Ledeaux

Manuel Rodriguez-Concepcion

Disclosed herein are zinc finger proteins that bind to target sites in a plant gamma-tocopherol methyl transferase (GMT) gene; compositions comprising these GMT-targeted zinc finger proteins and methods of making and using such zinc finger proteins. Also disclosed are methods for modulating the alpha-tocopherol content in various plant organs, particularly seeds, in transgenic plants.

Compositions and methods for regulation of plant gamma-tocopherol methyltransferase

Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.

Transgenic plants containing altered levels of steroid compounds

Provided are recombinant constructs comprising DNA sequences encoding enzymes effective in altering the biosynthesis and accumulation of sterol compounds and tocopherols in transgenic plants. Also provided are methods of using such constructs to produce transgenic plants, seeds of which contain elevated levels of sitostanol and/or sitostanol esters, and &#x3b1;-tocopherol, as well as reduced levels of campesterol and campestanol and their corresponding esters. These seeds also contain the novel sterol brassicastanol. Oil obtained from seeds of such transgenic plants is also provided. This oil can be used to prepare food and pharmaceutical compositions effective in lowering the level of low density lipoprotein cholesterol in blood serum. In addition, novel DNA sequences encoding plant steroid 5&#x3b1;-reductases are also disclosed.

Transgenic plants containing altered levels of sterol compounds and tocopherols

The present invention is in the field of plant biochemistry. More specifically the invention relates to nucleic acid sequences from plant cells, in particular, nucleic acid sequences from maize and soybean associated with the tocopherol synthesis pathway enzymes. The invention encompasses nucleic acid molecules that encode proteins and fragments of proteins. In addition, the invention also encompasses proteins and fragments of proteins so encoded and antibodies capable of binding these proteins or fragments. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression and transgenic plants.

Nucleic acid molecules associated with the tocopherol pathway

The present invention provides and includes nucleic acids, proteins and antibodies associated with novel genes in the MEP pathway. The invention further encompasses methods utilizing such molecules, for example in gene isolation, gene analysis and the production of transgenic plants. The present invention also includes transgenic plants modified to express proteins associated with the MEP pathway.

Methyl-D-erythritol phosphate pathway genes

Growing community of inventors

Ballwin, MO, United States of America

Mylavarapu Venkatramesh