Csir

Musa M Mhlanga

Jerolen Naidoo

Samantha Barichievy

Rethabile Khutlang

Stephanie Fanucchi

Youtaro Shibayama

Shaun Burd

Janine Scholefield

Emiliano Dalla

The present invention relates to methods for the identification of anti-HIV miRNAs and anti-HIV pharmaceutical compounds using high-throughput screening methods, comprising: transfecting reporter cells with a panel of miRNAs, infecting the reporter cells with HIV, screening the cells to identify miRNAs that modulate HIV infection and identifying the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs. The invention further provides for the identification and screening of anti-HIV pharmaceutical compounds having known activity against the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs for efficacy in the treatment of HIV. The invention also provides for the use of miRNA mimics, miRNA inhibitors and pharmaceutical compounds (including oncology drugs and kinase inhibitors) in the treatment and/or prevention of HIV infection.

Method for identification of anti-HIV human miRNA mimics and miRNA inhibitors and anti-HIV pharmaceutical compounds

This invention relates to a single cell assay for determining the effect of chromosomal contact on the transcriptional activity of genes of interest in a cell and to methods of silencing gene expression in a cell by way of perturbing gene regulatory elements which are engaged in chromosomal contact.

Site-specific nuclease single-cell assay targeting gene regulatory elements to silence gene expression

The present invention relates to methods and compositions for modulating the control of apoptosis, specifically for modulating the interaction between hnRNP-K and lincRNA-p21 to induce or prevent apoptosis in the cell.

Pathogenic control of apoptosis

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Johannesburg, South Africa

Musa M Mhlanga