UniversitÉ´de Rouen

Centre National De La Recherche Scientifique

Medicago Inc.

Institut Francais De Recherche Pour L'exploitation De La Mer (ifremer)

Algenics

L'institut Francais De Recherche Pour L'exploitation De La Mer (ifremer)

Muriel Bardor

Patrice Lerouge

Marc-Andre D'Aoust

Louis-Philippe Vezina

Véronique Gomord

Jean-Paul Cadoret

Marc-André D'Aoust

Stéphanie Aquin

Christophe Rihouey

Loïc Faye

Aude Carlier

Carole Burel

Thomas Paccalet

Stephanie Aquin

Florian Maury

Rémy Michel

Bérangère Baiet

Bruno Saint-Jean

Romain Louvet

Christophe Sourrouille

Marc-Andre D'aoust

Loiec Faye

Estelle Marquet-Blouin

A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.

Synthesis of sialic acid in plants

Transformedincluding a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an &#x3b1;-Mannosidase II and wherein at least one &#x3b2;-N-acetylglucosaminidase of the transformedhas been inactivated. A method for producing a glycosylated polypeptide includes the steps of (i) culturing a transformedas defined previously and (ii) purifying the polypeptide that is expressed and glycosylated in the transformed. The use of such a transformedfor producing a glycosylated polypeptide is also described.

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A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.

Transformed microalgae capable of expressing glycosylated polypeptides and methods for producing said transformed microalgae and producing glycosylated polypeptides.

Production of glycosylated polypeptides in micro algae

Growing community of inventors

Mont Saint Aignan, France

Muriel Bardor