Bigtec Private Limited

Mulakkapurath Narayanan Manoj

Pillarisetti Venkata Subbarao

Chandrasekhar Bhaskaran Nair

Madhuri Baliga

Kirubakaran Naveen Kumar

Venkata Ramachandra Rao Vasamsetty

Phani Kumar Pullela

Mitchell Preetham Pinto

Santhosh Kumar Gandhavalla

In one aspect, methods relate to the field of recombinant DNA therapeutics. Methods may involve bio-informatics design, synthesis of artificial genes for a human insulin precursor having a leader peptide coding sequence, cloning artificial genes into an expression vector, and expression in an organism such as one selected from the genus. In another aspect, methods may include downstream processing for obtaining protein precursor molecules and subsequent conversion of protein precursor molecules to functional proteins.

Method of downstream processing for conversion of human insulin precursor molecule into a functional human insulin protein molecule

Polynucleotide and polypeptide sequence and methods thereof

The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.

Method for isolation of nucleic acids and a kit thereof

The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

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Bengaluru, India

Mulakkapurath Narayanan Manoj