Indian Oil Corporation Limited

Department of Biotechnology

Mukund Adsul

Suresh Kumar Puri

Deepak Kumar Tuli

Ravi Prakash Gupta

Anshu Shankar Mathur

Simranjeet Kaur Sandhu

Reeta Rani Singhania

Sankara Sri Venkata Ramakumar

Jitendra Kumar Saini

The present invention relates to a process for simultaneous production of citric acid and cellulolytic enzymes. The batch process comprising (i) adding slurry of a pre-treated lignocellulosic biomass or cellulose in a fermentation media; (ii) inoculating 10% (v/v) active liquid seed culture ofMRJ-16 in the fermentation media of step (i); (iii) subjecting the culture of step (ii) to fermentation in an aerated fermenter at a temperature of 28-33° C. for a duration of 96 hours; and (iv) collecting enzyme broth after fermentation of step (iii) to obtain citric acid and cellulolytic enzymes. The batch and fed-batch process of the present invention results in high titer production of citric acid and cellulolytic enzymes in a single step and using single microbial strain.

Process for simultaneous production of citric acid and cellulolytic enzymes

The present invention relates to a mutant fungal strain of'MRJ-16' characterized by the ability to produce high titer of enzyme mixture comprising FPase, CMCase, Cellobiase, &#x3b2;-glucosidase, endoglucanase, &#x3b1;-L arabinofuranosidase, &#x3b2;-xylosidase, xylanase, pectinase, cellobiohydrase and oxidases and produce enzymes in the presence of a catabolite repressor molecule like glucose and/or xylose. The titer of enzyme mixture produced using mutant fungal strain MRJ-16 is at least two fold higher than naivestrain NCIM 1228, when used in a fermentation process. The mutant strain 'MRJ-16' with high hydrolytic activity and catabolite derepressed character is having application in the method of degrading or saccharifying biomass to produce valuable products for example-bioethanol.

Method for preparation and screening of fungal mutant with high hydrolytic activity and catabolite derepressed character

A method for preparing a hyper-cellulolytic catabolite derepressed mutants of ascomycetes fungus, especially variants of. Selection media used to isolate such variants include amorphous cellulose and a high concentration of glucose. Cellulase activities of mutant ID-10, in particular such as FPase and &#x3b2;-glucosidase were 1.5 times higher thanMRJ-16 (parent). Furthermore, fungal mutant morphology was changed and no pH adjustment was required throughout the enzyme production process.

Method for preparation of fungal mutant with high hydrolytic activity

The present invention relates to a method for obtaining a high titer of enzyme mixture comprising cellulases, hemicellulases and &#x3b2;-glucosidases in reutilization of waste water generated during hot water extraction of lignocellulosic biomass or biorefinery waste water usingMRJ-16 mutant strain. The cellulose or lignocellulosic biomass used in the fermentation process is selected from the group consisting of rice straw, wheat straw, corn stover, cotton stalk or a combination thereof. The enzyme mixture obtained by the present process is used for the saccharification of acid pretreated lignocellulosic biomass.

Bio-refinery waste utilization for enzyme production using novel penicillium funiculosum MRJ-16 fungal strain

The present invention relates to a method of cellulases enzymes production byMRJ-16 using minimum media components and/or low cost media components like cellulose or pretreated lignocellulosic biomass as carbon source and soya flour or defatted soya flour or de-oiled soya cake as nitrogen source. The present invention also provides a method for production of high titer of cellulases and hemicellulases enzymes usingMRJ-16 using minimum media and/or low cost media components.

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Faridabad, India

Mukund Adsul