Biocon Limited

Mukesh Babuappa Patale

Sanjay Tiwari

Harish Iyer

Anuj Goel

Kedarnath Nanjund Sastry

Nagaraj Govindappa

Anand Khedkar

Partha Hazra

Nitesh Dave

Mayank Kumar Garg

Chittnalli Ramegowda Naveen Kumar

Saurabh Garg

Sulekha Joshi

Krishnamurthy Venkatesan

Bimal Kumar

Nita Roy

Gokul Jothiraman

Goldy Sachdev

Komal N Kanojia

Nandini Nataraj

Madhavan Buddha

Vivekanandan Kannan

Anoop Vasudevan

Anupama Jagadish

Sebastian Alastair Mcdonald

Ranitendranath Tagore

Komal Kanojia

The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogs, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as

Fermentation medias and processes thereof

The present invention relates to novel Prolipase-Bovine trypsinogen (PLBTR) fusion proteins, the genes encoding them, and the production and uses thereof. More specifically, the present invention relates to methods of producing in optimal quantities PLBTR fusion proteins which comprise a heterologous polypeptide which is normally susceptible to autocatalytic activity. More particularly, the present invention relates to fusion proteins which comprise an heterologous polypeptide, such as a serine protease, fused to a lipase signal sequence, which can be expressed by recombinant host cells in desired amounts. The present invention further relates to polynucleotides encoding such fusion proteins, to expression vectors for expression of such fusion proteins, to host cells transformed with such polynucleotides/vectors, and to methods of generating such fusion proteins.

Fusion proteins and method of expression thereof

The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as

The invention relates to methods of separation and/or purification of impurities yielding a purified heterologous protein product devoid of related impurities or with substantially minimal quantities of such glycosylated impurities. More specifically, the invention relates to the identification of glycosylated forms of insulin analogues such as glargine impurities characterized post expression in yeast based systems such as. The invention also relates to methods used to clone gene encoding the protein insulin glargine; inserting the related gene in a suitable yeast host; producing culture of the recombinant strain, stimulating expression of the heterologous polypeptide, its secretion and purification post fermentation and related enzymatic conversions.

Method of obtaining a purified, biologically active heterologous protein

The disclosure relates to method of reducing O-glycosylation levels of the insulin or insulin analog precursor molecule produced bysp. The present disclosure provides genetically engineered knock-out strains of methylotrophic yeast includingand especiallyby disruption of Protein mannosyl transferase (PMT) genes and rendering them capable of producing heterologous proteins with reduced glycosylation. Vectors, which comprise coding sequences for PMT1, PMT2, PMT4, PMT5, and PMT6 genes, for transforming methylotrophic yeasts are contemplated by the present disclosure. PMT inactivated strains of this disclosure have been deposited at MTCC, Chandigarh. The strains are PMT1/GS115 (MTCC 5515), PMT4/GS115 (MTCC 5516), PMT5/GS115 (MTCC 5517) and PMT6/GS115 (MTCC 5518).

Method of reducing glycosylation of proteins, processes and proteins thereof

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Maharashtra, India

Mukesh Babuappa Patale