Inguran, LLC

Monsanto Technology LLC

Muhammad Anzar

Cindy L Ludwig

Jeffrey Alan Graham

Kathleen S Crowley

Gary Durack

Bradley Didion

Jeanette A Glaenzer

A method of cryopreserving sperm cells which may include cooling the quantity of sperm cells to a holding temperature, maintaining the sperm cells substantially at the holding temperature for a period, and supercooling the sperm cells by cooling the sperm cells at a first cooling rate to a temperature that approaches a critical temperature zone at which ice crystal formation and changes in osmotic pressure damage sperm cells and then cooling the sperm at a second cooling rate faster than said first cooling rate through said critical temperature zone.

Methods for processing sperm cells

A method of processing animal sperm cells that includes: collecting sperm cells from a male animal; sorting the sperm cells to obtain a quantity of sperm cells having a desired characteristic, the quantity of sorted sperm cells being contained in a first volume of fluid having a first concentration of sperm cells; and subjecting the sperm cells to concentration steps in which the concentration of the sperm cells is increased to a second concentration greater than the first concentration.

Sperm cell processing methods

Staining mixtures comprising viable spermatozoa, a composition which regulates oxidation/reduction reactions intracellularly or extracellularly, and a DNA selective dye are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for staining sperm cells comprising the formation of a staining mixture are also disclosed.

Use of a composition which regulates oxidation/reduction reactions intracellularly and/or extracellulary in a staining or sorting process

Sperm cells are stained according to processes that involve the combining of sperm cells with a fluorescent DNA selective dye at an elevated temperature in excess of about 40&#xb0; C. The methods allow for a decreased staining time. The cells may thereafter be efficiently sorted according to common separation methods, including flow cytometry.

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Guelph, Canada

Muhammad Anzar