Riken Corporation

Sumitomo Chemical Company, Limited

Sumitomo Dainippon Pharma Co., Ltd.

Sumitomo Pharma Co., Ltd.

Racthera Co., Ltd.

Mototsugu Eiraku

Yoshiki Sasai

Satoshi Ando

Tokushige Nakano

Takafumi Wataya

Masayo Takahashi

Atsushi Kuwahara

Satoru Okuda

Daiki Nukaya

Yuiko Kinose

Akishi Onishi

Hideya Sakaguchi

Provided is a method of inducing the differentiation of a stem cell into nerve progenitor cells, comprising the step (1) of forming homogenous aggregates of stem cells in a serum-free medium (1) and the step (2) of suspension-culturing the homogenous aggregates of stem cells in the presence of a basement membrane reference standard.

Method for differentiation induction in cultured stem cells

The present invention aims to provide a method for suppressing differentiation of ganglion cell, amacrine cell, horizontal cell and/or bipolar cell in a neural retina tissue containing photoreceptor precursor and/or photoreceptor, and the like. A method for suppressing differentiation of a ganglion cell, an amacrine cell, a horizontal cell and/or a bipolar cell in a neural retinal tissue containing a photoreceptor precursor and/or a photoreceptor, including a step of culturing a retinal tissue comprising a neural retinal progenitor cell and in any stage between a differentiation stage immediately after emergence of a ganglion cell and a differentiation stage where emergence rate of a cone photoreceptor precursor reaches maximum in a medium containing a thyroid gland hormone signal transduction pathway agonist.

Method for producing retinal tissues

The invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure by culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more of the tissue in a serum-free medium or serum-containing medium, each containing a substance acting on the Wnt signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the 'cell aggregate in which a RPE65 gene expressing cell does not appear' thus obtained in a serum-free medium or serum-containing medium, each not containing a substance acting on the Wnt signal pathway and so on.

Method for producing ciliary marginal zone-like structure

The invention provides a method of cryopreserving a tissue derived from a pluripotent stem cell, including the steps of (1) bringing a tissue derived from a pluripotent stem cell into contact with a cell protection solution that contains sulfoxide and chain polyol, (2) maintaining, in a cryopreservation solution, the pluripotent stem cell-derived tissue that was brought into contact with the cell protection solution in the first step, and (3) cryopreserving, in the presence of a coolant, the pluripotent stem cell-derived tissue that was maintained in the cryopreservation solution.

Method of cryopreservation of tissue derived from pluripotent stem cells

The invention provides a method for producing a retinal tissue by (1) subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) subjecting the aggregate to floating culture in a serum-free medium containing a basement membrane preparation, and then (3) subjecting the aggregate to floating culture in a serum-containing medium. The invention also provides a method for producing an optic-cup-like structure, a method for producing a retinal pigment epithelium, and a method for producing a retinal layer-specific neural cell.

Methods for producing retinal tissue and retina-related cell

The present invention enables efficient suspension culture of stem cells in a serum-free medium by comprising a step for quickly forming a homogenous aggregate of stem cells, and provides a method of selectively inducing the differentiation of nerves from a stem cell, a method of forming a cerebral cortical nerve network in vitro, and a method of producing a steric structure of a brain tissue in vitro, as well as a method of producing hypothalamic neuron progenitor cells, comprising culturing pluripotent stem cells as a suspended aggregate in a serum-free medium that substantially does not contain a Nodal signal promoter, a Wnt signal promoter, an FGF signal promoter, a BMP signal promoter, retinoic acid and an insulin, and isolating hypothalamic neuron progenitor cells from the culture.

Method for culture of stem cell

A specific region such as a continuous epithelial structure is automatically detected in a cell aggregate. A region detecting method includes an input step (S01) of inputting an image obtained by imaging a cell aggregate, an outline detecting step (S02) of detecting an outline of the cell aggregate together with an order along the outline from the input image, a region detecting step (S03) of detecting a specific region included in the cell aggregate based on the detected outline and order, and an output step (S04) of outputting information indicating the detected region.

Region detecting method and region detecting device related to cell aggregation

Provided is a method of inducing the differentiation of a stem cell into nerve progenitor cells, comprising the step (1) of forming a homogenous aggregate of stem cells in a serum-free medium (1) and the step (2) of suspension-culturing the homogenous aggregate of stem cells in the presence of a basement membrane reference standard.

Method for producing retinal tissue in vitro

The present invention enables efficient suspension culture of stem cells in a serum-free medium by comprising a step for quickly forming homogenous aggregates of stem cells, and provides a method of selectively inducing the differentiation of nerves from a stem cell, a method of forming a cerebral cortical nerve network in vitro, and a method of producing a steric structure of a brain tissue in vitro, as well as a method of producing hypothalamic neuron progenitor cells, comprising culturing pluripotent stem cells as suspended aggregates in a serum-free medium that substantially does not contain a Nodal signal promoter, a Wnt signal promoter, an FGF signal promoter, a BMP signal promoter, retinoic acid and an insulin, and isolating hypothalamic neuron progenitor cells from the culture.

Growing community of inventors

Wako, Japan

Mototsugu Eiraku