Hitachi-High-Technologies Corporation

Hitachi High-Tech Corporation

Nippon Precision Circuits Inc.

Applied Biosystems, LLC

Motohiro Yamazaki

Ryoji Inaba

Ryusuke Kimura

Takashi Anazawa

Taro Nakazawa

Takashi Gomi

Yoshitaka Kodama

Hiroyuki Higashino

Michiru Fujioka

Seiichi Ugai

Shuhei Yamamoto

Satoshi Takahashi

Kazumichi Imai

Mitsuhiro Miyazaki

Masaya Kojima

Hiromi Yamashita

Wataru Sato

Katsuhiro Aritome

Hitoshi Miyata

Ayaka Okuno

Kunio Harada

Hiroyuki Tanaka

Tomohiro Shoji

Yoshihiro Nagaoka

Kenko Uchida

Tomoyuki Sakai

Toru Yokoyama

Akira Fujii

Takeshi Ohura

Takayasu Furukawa

Jin Matsumura

Syozo Kasai

Yuichiro Ota

Yoshio Onodera

Takamichi Muramatsu

Noriyuki Shimoda

Nobuhiko Sato

Naomichi Kawasaki

Go Nakajima

Yuki Nonaka

Shigeru Matsui

Hiroyuki Noda

Masashi Watanabe

Eric S Nordman

Ai Masuda

Kiyotoshi Mori

Akira Toyama

Tomonari Morioka

Hideyuki Akiyama

Takeshi Ooura

Shintaro Kubo

Yoshiyuki Okishima

Kohshi Maeda

Miho Ozawa

Takuya Matsui

Hisao Inami

Akihiro Suzuki

Kosaku Toyosaki

Hidenori Namba

Yoshinori Ookoshi

Tsukasa Ohira

Shuichi Ando

Tatsuya Yamashita

Yasutoshi Morita

Katsunobu Hama

Isao Haraura

Yukari Sahoyama

Masashi Tomiyoshi

In an instrument configured to spectroscopically divide fluorescences emitted from a plurality of capillaries and collectively measure the fluorescences using an image sensor, when the number of pixels of a binning region on the image sensor on which a predetermined wavelength-band component of each fluorescence is projected is denoted by B, the number of pixels of hardware binning is denoted by B, the number of pixels of software binning is denoted by B, B=B×B, the total noise measured in a case where B=B=B=1 is denoted by N, the readout noise is denoted by N, the dark-current noise is denoted by N, and the shot noise is denoted by N, B, B, B, N, N, N, and Nsatisfy a predetermined relationship, thereby realizing high sensitivity and high dynamic range in fluorescence measurement.

Multi-capillary electrophoresis instrument

In an analysis method and an analysis system for detecting fluorescences from each of a plurality of light-emitting points in a plurality of wavelength bands in order to identify fluorescence emissions of a plurality of types of fluorophores from the plurality of light-emitting points, spatial crosstalk and spectral crosstalk are present between the plurality of light-emitting points and between the plurality of wavelength bands, and then performance of the identification is reduced. The spatial crosstalk and the spectral crosstalk are eliminated and concentrations of each of the plurality of types of fluorophores at each of the plurality of light-emitting points are derived by inputting all detection signals in the plurality of wavelength bands for the plurality of light-emitting points to a predetermined arithmetic operation expression.

Analysis system and analysis method

The present invention aims to provide an electrophoresis device which detects the presence or absence of foreign matter in a flow path. In order to solve the above problems, the electrophoresis device of the present invention is characterized by including a flow pathfilled thereinside with a migration medium, a migration medium container which stores a migration medium, a liquid feeding mechanism which feeds the migration medium to the flow path, a photographing devicewhich photographs the flow path, and a control unit which analyzes an image of the flow pathphotographed by the photographing deviceand in that the control unit determines the presence or absence of the foreign matter in the flow pathon the basis of the image of the flow path

Electrophoresis device and foreign matter detection method

Provided is an electrophoresis device that, by electrophoresis, feeds a sample into capillaries and optically detects the sample, the electrophoresis device being provided with capillaries, a capillary head provided at the distal end of the capillaries, a phoretic medium-filled container used for electrophoresis and filled with a phoretic medium, a guide member that covers the side surface of the phoretic medium-filled container, a seal member that seals from below the phoretic medium filled in the phoretic medium-filled container, and a plunger that presses the seal member.

Electrophoresis method

Provided is an electrophoresis device for enhancing its analysis performance. In order to achieve the task, the electrophoresis device includes a capillary array constituted by a capillary, a capillary head for bundling an end of the capillary, an electrode holder for holding an electrode provided at the other end of the capillary and a detector provided for the capillary, a first heater for heating the capillary, and an irradiation detection unit for irradiating a light to the detector to detect a fluorescence generated from a fluorescence labelled sample in the capillary. The electrophoresis device further includes a second heater for heating the detector.

Electrophoresis device

A capillary electrophoresis apparatus is disclosed that does not discharge and achieves both compactness and performance even with a part configuration having insufficient spatial distance or creeping distance. This capillary electrophoresis apparatus is provided with a resistance heater for heating capillaries, an electrode holder that holds capillary electrodes and is connected to a high-voltage unit, and a conductive member that at least partially comprises metal and has been grounded to a low potential. The electrode holder and conductive member are in contact with heat-dissipating rubber disposed there between that composes a structure comprising an insulation member. As a result of this configuration, discharge risk is reduced through the reduction of the potential of parts near the high-voltage unit and the slow reduction of the high potential of the high-voltage unit.

Capillary electrophoresis apparatus

The capillary electrophoresis apparatus according to the present invention can maintain the temperature in the longitudinal direction of each of a plurality of capillaries uniformly, such that the separation performance of the capillary electrophoresis apparatus can be stabilized, and the analysis performance can be improved. A capillary electrophoresis apparatus according to the present invention includes: a thermostat having a heat source, a first heat conduction member, and a detection window for detecting a sample, the thermostat being configured to maintain a capillary at a predetermined temperature; a capillary holder having a second heat conduction member for sandwiching the capillary between the second heat conduction member and the first heat conduction member, the capillary holder being configured to hold the capillary; and a detection unit configured to detect a sample to be electrophoresed in the capillary, wherein in the heat source, a heat generation amount of at least one of a periphery of the detection window and an end of the capillary is higher than a heat generation amount of another portion.

Capillary electrophoresis apparatus and thermostat

A capillary cartridge achieves both improvement of attachability and improvement of heat dissipation performance for realizing short-time analysis. A heat dissipation body is provided between a capillary having a detection unit provided in a part thereof and a plate-like support body that supports the capillary, and temperature increase inside the capillary is suppressed by the heat dissipation body, and thereby, electrophoresis can be performed under a high voltage application condition where the amount of heat increases and analysis time is reduced. In addition, it is possible to redress complexity of an operation by reducing a fixing place at the time of attachment to only a detection unit and an electrode holder by using an integration structure in which the capillary, the supporting body, and the heat radiating body are integrated.

Capillary cartridge and electrophoresis apparatus

A light-emission detection apparatus is provided for individually condensing light emitted from each emission point of an emission-point array using each condensing lens of a condensing-lens array to forma light beam and detecting each light beam incident on a sensor in parallel. The light-emission detection apparatus can be downsized and high sensitivity and low crosstalk can be simultaneously accomplished when a certain relation between the diameter of each emission point, a focal length of each condensing lens, an interval of condensing lenses, and an optical path length between each condensing lens and a sensor is satisfied.

Light-emitting detection device

The invention is directed to a light-emission detection apparatus for individually condensing light emitted from each emission point of a emission-point array using each condensing lens of a condensing-lens array to form a light beam and detecting each light beam incident on a sensor in parallel, and the light-emission detection apparatus can be downsized and high sensitivity and low crosstalk can be simultaneously accomplished when a certain relation between the diameter of each emission point, a focal length of each condensing lens, an interval of condensing lenses, and an optical path length between each condensing lens and a sensor is satisfied.

Provided are a bubble eliminating structure and a bubble eliminating method which eliminate bubbles in a liquid by agitating the liquid, and an agitating method using the same. A first groove, which is an upstream bubble eliminating groove, and a second groove, which is a downstream bubble eliminating groove, are branched from a mixing well. After starting suction of the liquid from mixing wellinto the first groove, suction of the liquid from the mixing wellinto the second grooveis started, and after completion of discharge of the liquid from the first grooveinto the mixing well, discharge of the liquid from the second grooveinto the mixing wellis completed. This operation is repeated to eliminate bubbles.

Bubble eliminating structure, bubble eliminating method, and agitating method using the same

In order to provide a piston and a syringe with good sliding performance and pressure resistance performance, the piston contained in the syringe that pressurizes or decompresses an internal medium is configured such that a soft portion made of a soft material and a rigid portion made of a material having rigidity higher than rigidity of the soft portion, are connected in series, and arranged such that the soft portion is in contact with the medium and the rigid portion is not in contact with the medium. The soft portion includes a hollow on a surface in contact with the medium. The rigid portion has an end surface facing the soft portion, the end surface including at least an outer peripheral portion in contact with the soft portion. The outer diameter of the soft portion is larger than the outer diameter of the rigid portion, and the outer diameter of the rigid portion is slightly smaller than the inner diameter of an outer cylinder of the syringe.

Piston and syringe

An electrophoresis device has: a sample tray () on which there are placed a positive-electrode-side buffer solution container () containing a buffer solution and a phoresis medium container () containing a phoresis medium, and which is driven in a vertical direction and a horizontal direction; a thermostat oven unit () that holds a capillary array having a capillary head in which a plurality of capillaries are bundled in a single unit at one end thereof in a state where the capillary array being held in a state in which the capillary head protrudes downward, and that keeps the interior temperature constant; a solution-delivering mechanism () for delivering the phoresis medium in the phoresis medium container to the capillary array from the capillary head; and a power source for applying a voltage to both ends of the capillary array. Holes for insertion of the capillary head are provided in upper sections of the positive-electrode-side buffer solution container and the phoresis medium container. The thermostat oven unit is provided with a first lid member () that is positioned above the sample tray and seals the upper section of the positive-electrode-side buffer solution container while the phoresis medium is being delivered by the solution-delivering mechanism.

Capillary electrophoresis device

The present invention addresses the problem of providing an electrophoretic medium container capable of stabilizing a liquid delivery pressure while ensuring sealing of an electrophoretic medium. In order to resolve this problem, this electrophoretic medium container is provided with a syringe part for holding an electrophoretic medium, and a sealing component for sealing one end of the syringe part, the electrophoretic medium container being characterized in that the sealing component has a sealing surface, a body part, and a groove provided between the sealing surface and the body part, wherein the sealing surface is in contact with the inner wall of the syringe part.

Electrophoretic medium container

Electrophoresis device and electrophoresis method

Growing community of inventors

Tokyo, Japan

Motohiro Yamazaki