National Institute of Advanced Industrial Science and Technology

National Institute of Technology and Evaluation

National Research Institute of Brewing

Tohoku University

Kikkoman Corporation

Amano Enzyme Inc.

Kanazawa Institute of Technology

Noda Institute for Scientific Research

Motoaki Sano

Masayuki Machida

Keietsu Abe

Katsuya Gomi

Kiyoshi Asai

Naotake Ogasawara

Hideki Nagasaki

Osamu Akita

Akira Hosoyama

Taishin Kin

Satoru Kuhara

Kenichiro Matsushima

Shotaro Yamaguchi

Yasuji Koyama

Tsutomu Masuda

Katsuhiko Kitamoto

Kotaro Ito

Atsuki Toumoto

Genryou Umitsuki

Akira Yoshimi

Osamu Hatamoto

Kensuke Yuuki

Manabu Arioka

Touru Ito

Ken Miyazawa

Fuka Tabata

Tasuku Nakajima

Misao Sunagawa

Satoru Hisahara

Genryou Umitsüki

Akimitsu Tanaka

Chitoshi Oka

Hiroshi Maeda

Hitoshi Tainaka

Tomomi Uchikawa

Tomomi Ichikawa

Provided is a mutant filamentous fungus, which lacks expression of &#x3b1;-1,3-glucan, and is deficient in at least part of a GAG biosynthetic cluster. Also provided is a method of producing a substance, including the steps of: culturing the filamentous fungus to allow the filamentous fungus to produce a substance; and collecting the resulting substance.

Mutant filamentous fungus and substance production method in which said mutant filamentous fungus is used

To improve the activity of a Koji mold protease in a solid or liquid culture medium in the production of foods (e.g., a seasoning), pharmaceuticals (e.g., a digestive agent), protease for use in a detergent and the like. Disclosed are a recombinant vector having capability of increasing the secretion of the Koji mold protease, a Koji mold which is transformed with the vector and has an increased expression of a gene for a protease or an increase secretion of the same, a method for the production of a protease by using the transformed Koji mold, and the like.

Recombinant vector capable of increasing secretion of Koji mold protease

It is intended to provide koji mold-origin phospholipase Aand a DNA encoding it. Namely, phospholipase Acomprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A.

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Lysyl oxidase derived from filamentous fungi and a DNA encoding thereof are provided. Lysyl oxidase including a protein described in of the following (a) or (b): (a) a protein having an amino acid sequence set forth in SEQ ID NO: 2; or (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as lysyl oxidase.

Fungus-origin lysyl oxidases

The purpose of the present invention is to isolate a gene encoding novel exo-, or endo-&#x3b2;--glucanase, to obtain a microorganism having an enhanced expression of said gene and to degrade &#x3b2;--glucan to its low molecular weight form by means of said enzymes. The present invention relates to a gene or DNA encoding novel enzymes having &#x3b2;--glucanase activity (exo-, or endo-&#x3b2;--glucanase), a recombinant expression vector comprising them, a microorganism having the recombinant expression vector, the novel enzymes having &#x3b2;--glucanase activity, and a method for the production of said enzymes.

Genes of cell wall-degrading enzymes derived from aspergillus, and method for the production the enzymes

A completely novel glutaminase is provided: (a) a protein consisting of an amino acid sequence represented by SEQ ID NO: 2, or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion, substitution or addition of one or more amino acids, and possessing glutaminase activity. This protein is a novel glutaminase possessing excellent thermostability and the like.

Thermostable glutaminase and thermostable glutaminase gene

Growing community of inventors

Tsukuba, Japan

Motoaki Sano