The Children's Hospital of Philadelphia

University of Pennsylvania

Mortimer Poncz

Douglas B Cines

Alan M Gewirtz

Deborah French

Paul Gadue

Mitchell Weiss

Compositions and methods for generating platelets and methods of use thereof are disclosed.

Compositions and methods for the generation of platelets and methods of use thereof

Recombinant nucleic acid molecules are constructed with a first sequence encoding a transgene under the control of regulatory sequences that direct expression of the transgene product in a hematopoietic stem cell, or a progenitor cell therefrom or cell differentiated therefrom. In one embodiment, the cell which expresses the transgene is a secretory cell. The cell is a megakaryotic progenitor cell, or a cell further differentiated therefrom, such as a platelet. The cell is a granulocyte/macrophage progenitor cell or a cell further differentiated therefrom, such as a mast cell or neutrophils. Such host cells containing the molecule or the molecule itself are employed in methods for treating or preventing infection, inflammation or vascular injuries or any disorders involving or mediated by cells of the hematopoietic lineage.

Delivery vehicle for recombinant proteins

Neutrophil activating peptide-2 or analog thereof is administered to a mammal to achieve therapeutic reduction of the number of circulating platelets. The peptide is useful in treating essential thrombocythemia and reactive thrombocytosis.

Growing community of inventors

Wynnewood, PA, United States of America

Mortimer Poncz