Snow Brand Milk Products Co., Ltd.

Morimasa Tanimoto

Shunichi Dosako

Yasuharu Itagaki

Kaoru Sato

Tadashi Idota

Yoshihiro Kawasaki

Hiroko Isoda

Mio Imamura

Hiroshi Kawakami

Yoshihiko Honda

Seiji Kurosawa

Shunichi Dousako

Klaas Doesburg

Yoshikiko Honda

Tetsuro Ohba

Shinichi Dosako

Taisuke Iwasaki

Jan Sikkema

A novel primer is provided which is useful in the reverse transcription polymerase chain reaction to amplify &agr;,-casein gene and keratin gene contained in milk. A method is also provided for using the expression ratio of their genes to examine the pathological conditions of mammalian mastitis or determine the milk protein synthesis level in mammary gland cells. The method is quick and accurate and avoids the need for excising mammary gland tissue.

Method for determining mastitis using keratin and casein which are amplified by primers

The present invention relates to a method of synchronizing epithelial cells into G.sub.0 phase, more specifically, a method of synchronizing epithelial cells into G.sub.0 phase characterized by culturing the cells in a synthetic medium for 5 days or more, wherein said synthetic medium is a medium with the lowered concentration of bovine fetal serum 5% or less and said epithelial cells is bovine mammary gland cells. Further, the present invention relates to a method of culturing cells characterized by using G.sub.0 phase synchronized cells obtained by the present invention, and a method of screening a related to differentiation and/or maturation of said cells by using G.sub.0 phase synchronized cells. The present invention is useful for easily synchronizing epithelial cells into G.sub.0 phase and for screening a factor related to differentiation and/or maturation of epithelial cells.

Method of synchronizing epithelial cells into G.sub.0 phase

This invention provides compositions for fair-skin containing the phosphorylated polysaccharides produced by lactic acid bacteria as an effective ingredient. The phosphorylated polysaccharides are expressed, for example, by the following formula and contained in the compositions for fair-skin at concentrations of 0.01-20 weight % and are used such as creams, emulsions and toilet lotions. The compositions for fair-skin exhibit fair-skin effect by inhibiting activities to melanoma proliferation and melanin formation. ##STR1## (wherein, Glc, Gal and Rha represent glucose, galactose and rhamnose residues, respectively. Numerals shown by other than subscript represent the respective binding sites and n represents the degree of polymerization of 1,000-5,000).

Compositions for fair-skin

Sialic acid binding proteins, sialic acid-binding peptides and sialic acid containing oligo-saccharides are active ingredients of a novel bacterial enterotoxin neutralizer and interfere with the binding of enterotoxins, including cholera toxin, to receptors so as to exert an effect of neutralizing the toxicity, thus being utilized as an effective neutralizer and are safe and available cheaply and abundantly from by-products produced during the processing of cow's milk which renders the enterotoxin neutralizer economically advantageous for commercial use.

Bacterial toxin neutralizer

A rotary column is rotatably mounted on a hollow shaft having orifices and the shaft is journaled in a reaction vessel. The rotary column has an outer net around the shaft and a space defined by this outer net within the rotary column is divided by partition plates provided with many through-holes into several compartments to be loaded with carriers and immobilized enzymes. The reaction vessel is provided radially outside the compartments with a top cover so that carriers and immobilized enzymes may be easily and evenly loaded into the rotary column. An end surface of the rotary column that is remote from the drive mechanism is provided with a lateral cover. There are provided hydraulic cylinders to tilt the rotary column. Within the reaction vessel, the outer net of the rotary column is provided with rollers adjacent to the lateral cover. Furthermore, there is provided a deaerating pipe within the rotary column and the orifices of the hollow shaft are so dimensioned that, nearer a solution outlet, the diameters of the orifices are larger.

Rotary column reactor

Disclosed is a method for separating and recovering very small amounts of biologically active proteins (such as enzymes, antigens and antibodies) present in a various fluids, with a high recovery and on a large scale, in which a fluid containing a desired protein is introduced into a rotary column through orifices therein and brought into contact with a gel carrier capable of selectively adsorbing the desired protein, while the carrier is being gentle fluidized in a liquid by the rotary motion successively by wash water and eluting fluid, so that the column, after which the fluid is displaced, the carrier having the desired protein adsorbed thereon is washed and the desired protein is recovered therefrom by elution while the carrier fluidized.

Fluidized bed separation and recovery of proteins from fluids in a

Method for separating and purifying lactoferrin from milk by use of

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Sayama, Japan

Morimasa Tanimoto