Shimadzu Corporation

Morimasa Hayashi

Yosuke Iwata

Nobuyuki Tatsumi

Yusuke Osaka

Yoshihiro Hayakawa

In order to obtain a temperature gradient elution method which is rapid and accurate, a mobile phase () is supplied through two mobile-phase flow channels () and (), mixed together, and introduced into the column () while the column () is made adiabatic. In this operation, the temperature of the mobile phase in one mobile-phase flow channel, i.e., the channel (), is regulated to a constant temperature higher than the upper limit of the target temperature range to be obtained in the column (), while the temperature of the mobile phase in the other mobile-phase flow channel, i.e., the channel (), is regulated to a constant temperature lower than the lower limit of the target temperature range in the column (). By controlling the flow rates in the two mobile-phase flow channels () and (), the proportion in which these mobile-phase portions are mixed is changed with time to thereby change the temperature of the mobile phase in the column () with time. The temperature gradient elution method is thus conducted.

Method and apparatus for analysis by liquid chromatography

A function of automatically performing a pretreatment process on a sample is provided in an auto sampler of a total volume injection method that can realize high measurement accuracy even when an amount of the sample is small. A multi-port valve and a multi-position valve are connected to each other, and a plurality of kinds of cleaning solutions and also a mobile phase are connected to the multi-port valve. By providing a certain volume in a tube connected to a measuring pump for suctioning the sample, the pump can suction a sufficient amount of the cleaning solution or a diluent solution at one suction operation. Consequently, an apparatus according to the invention can deal with the pretreatment such as cleaning and dilution, without repeating the same suction/discharge operations.

Automatic sample introduction apparatus

A two-dimensional liquid chromatograph includes a first-dimension separation channel for guiding a sample injected from a sample injection part to a first-dimension analysis column using a first-dimension analysis mobile phase for separation; two trap columns; an analysis channel for guiding components retained in the trap columns to a second-dimension analysis column using a second-dimension analysis mobile phase for analysis; and a channel switching mechanism. The switching mechanism connects the first-dimension separation channel to one of the trap columns and connecting the analysis channel to the other of the trap columns, and also switches connections between the trap columns and the channels.

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Kyoto, Japan

Morimasa Hayashi