Macquarie University

Btf Pty Ltd.

Moreland David Gibbs

Peter Bergquist

Graham Vesey

Peter Leonard Bergquist

Leonardo B Pinheiro

Kaisu Milja Helena Nevalainen

Rosalind Reeves

An isolated mutated green fluorescent protein (gfp) gene for chromosomal insertion into a bacterium, wherein the gene is capable of being expressed in bacteria and produce sufficient fluorescence under illumination from a UV lamp in a bacterial colony to be seen by the naked eye. A gene cassette for inserting a gene into a chromosome.

Chromosomal insertion of GFP into bacteria for quality control

A method for gene shuffling to form a mutant or chimeric gene, the method comprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3&#x2032; degenerate ends.

Degenerate oligonucleotide gene shuffling

A method for producing a mutant gene encoding a functional gene product comprising: (a) introducing one or more mutations into a gene to form a mutated gene; (b) providing the mutated gene to a host microorganism; (c) culturing the host microorganism containing the mutated gene under conditions which allow expression of the mutant gene product; and (d) selecting a host microorganism capable of producing a functional gene production from the mutated gene.

Random drift mutagenesis

Growing community of inventors

Marsfield, Australia

Moreland David Gibbs