Pacific Biosciences of California, Inc.

Omniome, Inc.

Morassa Mohseni Middleton

Arnold Oliphant

Brittany Ann Rohrman

Pinar Iyidogan

Fabian Block

Ying Lin Liu

Mark C Wallen

Michael James Schmidt

Denis A Malyshev

Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca. In addition to Li, the fluid can contain polyethylenimine (PEI) with or without betaine.

Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes

A method for identifying a nucleotide in a primed template nucleic acid, including the steps of (a) providing a vessel having a primed template nucleic acid, polymerase and a nucleotide cognate of a first base type; (b) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the first base type bound at a base position of the primed template nucleic acid; (c) delivering a nucleotide cognate of a second base type to the vessel, whereby the vessel retains the primed template nucleic acid and the polymerase from step (b); (d) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the second base type bound at the base position of the primed template nucleic acid; and (e) identifying the type of nucleotide at the base position of the primed template nucleic acid.

Serial formation of ternary complex species

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San Diego, CA, United States of America

Morassa Mohseni Middleton