Bioleaders Corporation

Kookmin University

Korea Research Institute of Bioscience and Biotechnology

The Industry & Academic Cooperation in Chungnam National University

Takara Bio Inc.

Korea Institute of Science and Technology

Industry-university Cooperation Foundation Sogang University

Chosun University

M.d. Lab Co., Ltd., Department of Veterinary Science Chung Nam University

Chungbuk National University Industry Academic Cooperation

Moon-Hee Sung

Seung-Pyo Hong

Chul-Joong Kim

Haryoung Poo

Seung-Goo Lee

Chang-Min Jung

Yong Taik Lim

Chul Joong Kim

Jong-Su Lee

Il Han Lee

Jae Su Song

Kwang Soo Kim

Seung Pyo Hong

Kyung-Soo Hahm

Ha Ryoung Poo

Yeon-Gyu Yu

Yoon Kyung Park

Jin-Woo Bae

Su-Mi Kim

Dae-Joong Kang

Sung-Keun Rhee

Che Ok Jeon

Young-Ki Choi

Hwa-Jung Seo

Dong-Gun Lee

Hyun-mi Pyo

Sue-nie Park

Young-Woock Noh

Joong-Jae Kim

Ha-Ryoung Poo

Hyun Min Kim

Ji Yeon Kim

Tae-Woo Kim

Jong-Soo Lee

Chung Won Park

Il Ha Lee

Kenji Soda

Makoto Ashiuchi

A Ri Jang

Hae-Jin Rhee

Sang-Mu Shim

Young Suk Kim

Dong Jin Jeong

Dai-Won Yoo

Seong-Pyo Hong

Long Chun Xu

Chang Min Jung

Jong Soo Lee

Eui-Jin Kim

Jeong-Kug Lee

A method of stabilizing a membrane protein includes forming a membrane protein-amphiphilic polymer complex by binding a membrane protein in an aqueous solution to the amphiphilic polymer represented by Formula 1. The amphiphilic polymer includes a large amount of hydrophilic structures and hydrophobic structures, and thereby effectively stabilizing a membrane protein having a hydrophobic surface in an aqueous solution.

Amphiphilic polymer

The present invention relates to an amphiphilic polymer which includes a large amount of hydrophilic structures and hydrophobic structures, and thereby effectively stabilizing a membrane protein having a hydrophobic surface in an aqueous solution. A method of preparing an amphiphilic polymer represented by Formula 1 includes reacting a poly-gamma-glutamic acid with a reaction product of a fluorescent dye, biotin, an alkyl carboxylic acid having 1 to 10 carbon atoms or a cycloalkyl carboxylic acid having 5 to 20 carbon atoms having a carboxyl group, and dicyclo-hexylcarbodiimide (DCC) and reacting the poly-gamma-glutamic acid with DCC after reacting the poly-gamma-glutamic acid with the reaction product, and reacting the poly-gamma-glutamic acid with a hydrophilic amine and a hydrophobic amine.

The present invention relates to an optical imaging probe for detecting a sentinel lymph node, which contains a complex of poly-gamma-glutamic acid and an optical imaging dye, and more particularly to an optical imaging probe for detecting a sentinel lymph node, which contains a poly-gamma-glutamic acid/optical imaging dye complex that, when injected subcutaneously in vivo, remains in the sentinel lymph node for a relatively long period of time and has a low tendency to migrate to other lymph nodes. The present invention provides a poly-gamma-glutamic acid/fluorescent dye complex, which contains no radiopharmaceutical and is harmless to the human body. The use of the poly-gamma-glutamic acid/fluorescent dye complex makes it possible to accurately detect the position of a sentinel lymph node in real time without concerns about radioactive contamination.

Optical-imaging probe for detecting sentinel lymph nodes which contains a composite of poly-gamma-glutamic acid and an optical-imaging dye

The present invention relates to an adjuvant composition containing poly-gamma-glutamic acid-chitosan nanoparticles and a vaccine composition containing the adjuvant composition, and more particularly to an adjuvant composition containing nanoparticles prepared by ionic bonding between poly-gamma-glutamic acid having ensured safety and chitosan, and a vaccine composition containing the poly-gamma-glutamic acid-chitosan nanoparticles and an antigen. The adjuvant containing the poly-gamma-glutamic acid-chitosan nanoparticles has little or no toxicity and side effects and is added to human or animal vaccines for the prevention and treatment of viral and bacterial infections and cancers to increase the production of antibodies.

Adjuvant composition containing poly-gamma-glutamic acid-chitosan nanoparticles

A vector for constitutively expressing a high level of a target protein, and more particularly a RepE mutant protein containing a deletion of 21 amino acids in the C-terminal region of a RepE protein and a vector for constitutively expressing a high level of a target protein, which comprises a gene encoding the mutant protein. The constitutive high-level expression vector can stably express a high level of a target protein. Also, the surface expression vector can express a target protein on the surface of recombinant microorganisms while constitutively expressing a high level of the target protein, and thus will be useful for construction of an antigen for vaccines.

Vector for constitutive high-level expression containing REPe mutant gene

The present invention relates to a nano-composite containing anionic and cationic polymers and a method for preparing thereof, and more particularly to a poly-gamma-glutamic acid (&#x3b3;-PGA)/chitosan/manganese iron oxide nanoparticle composite prepared by encapsulating iron oxide-based nanoparticles in a &#x3b3;-PGA/chitosan polymer composite using the ionic self-assembly properties of poly-gamma-glutamic acid (&#x3b3;-PGA) and chitosan, which are biocompatible polymer materials, and to a method for preparing thereof. The present invention provides a magnetic resonance imaging nano-contrast agent based on a nanoparticle composite including iron oxide-based nanoparticles encapsulated in a self-assembled composite of anionic poly-gamma-glutamic acid (&#x3b3;PGA) and cationic chitosan. The iron oxide-based nanoparticles encapsulated in the composite interact with each other to produce a synergistic effect on the amplification of magnetic resonance signals, and thus have an relatively excellent contrast effect compared to when they exist as single particles.

High-sensitivity magnetic resonance imaging nano-contrast agent based on an anionic polymer and cationic polymer ion complex, and a production method therefor

The present invention relates to a pharmaceutical composition for inhibiting or preventing viral infection, which comprises an effective dose of poly-gamma-glutamic acid (&#x3b3;-PGA), and more particularly, to a pharmaceutical composition, a functional food, and a feedstuff additive capable of inhibiting viral infection and preventing viral diseases, which comprise poly-gamma-glutamic acid having an infection-inhibiting effect against viruses, such as an influenza virus, inducing respiratory infection or systemic infection, as an effective ingredient. The composition containing poly-gamma-glutamic acid as an effective ingredient according to the present invention, is effective for use as an animal feedstuff additive or a pharmaceutical agent for preventing influenza virus infection and various viral diseases as well as a pharmaceutical composition and a functional food to promote human health.

Composition for preventing virus infection comprising poly-gamma-glutamic acid

A surface expression vector for preparing HPV immunogenic compositions, in which the surface expression vector contains a gene encoding a repE mutant protein having an amino acid sequence of SEQ ID NO: 1, a promoter, a poly-gamma-glutamate synthetase complex gene, and a gene which is linked with the poly-gamma-glutamate synthetase complex gene and encodes a tumor induction-associated antigen protein of human papillomavirus. An expression vector constitutively expressing a high level of the human papillomavirus (HPV) antigen protein is provided. Also, a recombinant lactic acid bacteria, transformed with the expression vector and expressing the HPV antigen protein on the surface thereof, and a composition comprising the recombinant lactic acid bacteria are provided. The recombinant lactic acid bacteria and the composition are very effective as a immunogenic composition for the treatment of cervical cancer, because they can be applied orally or directly to the vagina.

Stable constitutively high expression vector for preparing HPV vaccine and recombinant lactic acid bacteria transformed thereby

The present invention relates to an anticoagulant and a composition for preventing thrombus formation, which contain poly-gamma-glutamic acid (PGA) as an active ingredient. The inventive PGA is a water-soluble, anionic, biodegradable and edible amino acid polymer material, which has an anticoagulant effect of preventing thrombi from being accumulated in blood vessels, shows an excellent sustained-release effect and is harmless to the human body. Thus, it is useful as a high-value-added anticoagulant and a food or beverage composition for preventing thrombus formation.

Anticoagulant and composition for preventing thrombus containing poly-gamma-glutamic acid

The present invention relates to a fusion protein in which a myostatin mature protein is fused to a multimer of myostatin-derived antigenic peptide Myo-2, a surface expression vector containing a polynucleotide encoding the fusion protein, a recombinant microorganism transformed with the vector, and a feedstuff additive or a pharmaceutical composition containing the microorganism as an effective ingredient. The feedstuff additive or pharmaceutical composition according to the present invention can be used for muscle development and regulation of muscle growth in livestock and poultry, as well as for preventing and treating muscle-wasting diseases and degenerative diseases such as muscular dystrophy, muscular atrophy and the like. In addition, the transformed strain shows the same effect even if the strain itself after culture thereof is directly used, and thus it is very economical.

Surface expression vector for fusion protein of Myo-2 peptide multimer and myostatin, and microorganism transformed by thereof

The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived fromsp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal 3 35.

Surface expression method of peptides P5 and Anal3 using the gene encoding poly-gamma-glutamate synthetase

Disclosed is a method for screening an antioxidant using mutant bacteria and chlorophyllide, and an antioxidant screened by the same method. The method provides for screening of an antioxidant by monitoring growth profiles of specific mutant bacteria in filter discs or medium blocks containing chlorophyllide added thereto, and an antioxidant screened by the same method. By monitoring growth profiles of mutant bacteria using mutant bacteria and chlorophyllide, screening of an antioxidant is possible on an industrial scale. In particular, the antioxidant screening method is useful for selective screening of an amphiphilic antioxidant. Therefore, it is possible to screen and commercialize low-toxic and effective antioxidants used in various food and cosmetic additives, as well as therapeutic medicines.

Method for screening antioxidant using mutant bacteria and chlorophyllide, and antioxidant screened by the method

Expression vectors that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-&#x3c7;-glutamic acid synthetase complex (pgsBCA) of asp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus. Methods for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.

Vector for anti-HPV vaccine and transformed microorganism by the vector

The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.

Surface expression vectors having pgsBCA the gene coding poly-gamma-glutamate synthetase, and a method for expression of target protein at the surface of microorganism using the vector

To provide a promoter, a recombinant DNA, a gene expression vector, an expression vector, and a transformant, which are capable of expressing a gene without inducing gene expression with an inducer; and a method for producing a protein and a kit therefor, which can be operated easily and performed inexpensively by convenient and inexpensive steps. An isolated DNA having the nucleotide sequence of SEQ ID NO: 1 or 2 of Sequence Listing or a fragment thereof, wherein the isolated DNA exhibits a constitutive promoter activity inor a bacterium belonging to the genus; an isolated DNA having a nucleotide sequence of a nucleic acid capable of hybridizing to the above DNA, wherein the isolated DNA exhibits a constitutive promoter activity inor a bacterium belonging to the genus; a recombinant DNA comprising the above DNA and a foreign gene, wherein the foreign gene is operably located; a gene expression vector, at least comprising the above DNA; an expression vector comprising the recombinant DNA; a transformant having the above recombinant DNA, or the above expression vector; a method for producing a protein, characterized by culturing the above transformant, and collecting a protein from the resulting culture; and a kit for producing a protein, at least comprising the above DNA, or the above gene expression vector.

Promoters and gene expression method by using the promoters

Growing community of inventors

Daejeon, South Korea

Moon-Hee Sung