Grifols, S.a.

Grifols Worldwide Operations Limited

Fundacio Institut D'investigacio Biomedica De Bellvitge (idibell)

Montserrat Costa Rierola

Juan Ignacio Jorquera Nieto

Pere Ristol Debart

Salvador Grancha Gamon

Ana Maria Ortiz Fernandez

Samuel Ojosnegros Martos

Jose Maria Diez Cervantes

José María Diez Cervantes

Anna Seriola Petit

Ana Maria Ortiz Fernández

José Maria Diez Cervantes

Ma Isabel Bravo Camison

A method prepares a solution of human albumin by reducing dissolved oxygen in a solution of albumin until a concentration of oxygen equal to or less than 0.5 ppm is achieved. A solution of human albumin having a redox state closer to the redox state of the albumin present in human plasma can be achieved.

Method for the preparation of human albumin with reduced level of dissolved oxygen

The present invention relates to mammalian cell culture media which comprise supernatant from some of the fractions of human plasma fractionation according to the Cohn method, more specifically, the supernatant of fractions I and II+III. When said supernatant is added as a culture medium supplement it provides various nutrients and factors for the effective maintenance and/or proliferation of the cultured mammalian cells. In addition, the present invention relates to the preparation process and use of said medium in the culture of mammalian cells.

Mammalian cell culture media which comprise supernatant from cohn fractionation stages and use thereof

A process for detoxifying the blood of a patient in need thereof involves: collecting albumin from human plasma and/or plasma fractions; diafiltration of a mixture containing the collected albumin; stabilizing the resulting diafiltered albumin mixture with sodium chloride and an amino acid in the absence of any added fatty acid; pasteurizing the stabilized albumin mixture; diafiltering the pasteurized mixture to remove, among other things, stabilizers; and detoxifying the blood of the patient by administering the resulting albumin to the patient. In one embodiment, the amino acid stabilizing agent is acetyl tryptophan. The resulting albumin has at least about 80% albumin binding capacity relative to natural plasma albumin, and is storage stable for about 30 months at 30&#xb0; C.

Process for obtaining high efficiency human albumin for use in detoxification therapy

A process for purifying and collecting albumin from plasma and plasma fractions involves diafiltration of an albumin-containing plasma fraction; stabilizing the resulting albumin fraction with sodium chloride and an amino acid in the absence of any added fatty acid; pasteurizing the stabilized albumin fraction; and diafiltering the pasteurized fraction to remove, along other things, stabilizers. In one embodiment, the amino acid stabilizing agent is acetyl tryptophan. The resulting albumin has at least about 80% albumin binding capacity relative to natural plasma albumin, and is storage stable for about 30 months at 30&#xb0; C.

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Montserrat Costa Rierola