Lfb Biotechnologies

Gencell, S.a.

Laboratoire Francais Du Fractionnement Et Des Biotechnologies

Monique Ollivier

Pierre Wils

Michel Nogre

Philippe Paolantonacci

Alain Lejars

The invention relates to a method for extracting a protein from milk, having at least one hydrophobic pocket and a negative charge to the natural pH of milk, that comprises the following steps: a) skimming and delipidation of the milk; b) passing the delipidated and skimmed fraction containing the protein on a chromatographic substrate on which is grafted a ligand having both a hydrophobic characteristic and an ionic characteristic in pH conditions enabling the protein to be retained on the substrate, the pH being higher than 4.6; c) elution of the protein; d) purification of the eluted fraction by removing the milk proteins from the eluted fraction; and e) recovering the protein.

Method for extracting a protein from milk

The invention is directed to a process for purifying double-stranded DNA, comprising using ceramic hydroxyapatite column chromatography. The invention is also directed to a purified recombinant plasmid DNA composition, comprising a chromosomal DNA content that is less than or equal to 0.01% as well as to a composition comprising DNA prepared by the foregoing process.

Purification of plasmid DNA of pharmaceutical quality

Growing community of inventors

Le Kremlin Bicetre, France

Monique Ollivier