Terumo Bct, Inc.

Monique Givens

Glen Delbert Antwiler

David A Windmiller

Brian J Nankervis

Jon A Dodd

Casey V Medina

Thomas G Dilorenzo

Michael E Kinzie

Edward Allan Stanton, Iv

Edward Allan Iv Stanton

This invention is directed to methods of directly reseeding harvested adherent cells grown in a hollow fiber bioreactor. Also disclosed is a novel harvest media for use in directly reseeding adherent cells into a hollow fiber bioreactor.

Method of reseeding adherent cells grown in a hollow fiber bioreactor system

Embodiments described herein generally relate to methods and systems for using an air removal chamber as a control for a process in a cell expansion system. The air removal chamber may be mounted on a fluid conveyance assembly for use with the system. Fluid is pumped into a fluid containment chamber of the air removal chamber, in which the level of fluid in the fluid containment chamber may be monitored through the use of one or more sensors. The sensors are capable of detecting air, a lack of fluid, fluid, and/or a gas/fluid interface, e.g., an air/fluid interface, at measuring positions within the air removal chamber. Protocols for use with the system may include one or more stop conditions. In an embodiment, the stopping of a process is automated based on the detection of air, a lack of fluid, and/or a gas/fluid interface in the air removal chamber.

Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions

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Westminster, CO, United States of America

Monique Givens