Fluid-screen, Inc.

Other

Monika Weber

Slawomir Antoszczyk

Robert Weber

Mark A Reed

Siu Lung Lo

Hazael Fabrizio Montanaro Ochoa

Christopher Daniel Yerino

Methods and apparatus for detecting, quantifying, enriching, and/or separating bacterial species in fluid sample are provided. The fluid sample is provided as input to a microfluidic passage of a microfluidic device, wherein the microfluidic device comprises at least one electrode disposed adjacent to the microfluidic passage. The at least one electrode is activated to capture bacteria in the sample using dielectrophoresis, wherein the capture efficiency of bacteria is at least 99%.

Methods and apparatus for separating live from dead organisms in a sample

Systems and methods are provided herein for rapid detection, separation, purification, and quantification of viral particles in a sample. According to some embodiments, a microfluidic device is provided for receiving the sample which may contain viral particles. An electrode of the microfluidic device may be used to generate dielectrophoretic (DEP) and/or electroosmotic (EO) forces acting on the sample. The applied DEP and/or EO forces may immobilize components of the sample on the surface of the electrode, may aggregate viral particles of the sample in one region of the microfluidic device, and may separate other components of the sample from the viral particles. The techniques may be performed rapidly, for example, in eight hours or less, and may not affect infectivity of the viral particles. In some embodiments, the sample may be labeled to enhance a response of one or more of the sample components to the DEP and/or EO forces.

Apparatus and methods to rapidly detect, separate, purify, and quantify various viruses from cells, cultured medium and other fluids

An apparatus for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The electric field is applied to at least one electrode having a plurality of concentric rings or concentric arcs extending radially outwards from a center point, electrically connected to a voltage source such that when voltage is applied to the at least one electrode, the concentric rings or concentric arcs alternate in voltage potential.

Apparatus for pathogen detection

Bacteria separation system and methods

Methods and apparatus for detection and/or identification of analytes including bacteria using dielectrophoresis and electroosmotic traps. Switching between different frequencies of an applied electric field results in movement of the analyte between dielectrophoresis and electroosmotic trapping states. The use of edge-based sensing techniques enables the use of electrodes with a larger form factor than nanowire sensors. Signal modulation based on analyte contact with the electrode edge is also described.

Analyte detection methods and apparatus using dielectrophoresis and electroosmosis

Bacteria enrichment system and methods

High-efficiency bacteria capture and quantification system and methods

An apparatus and method for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. Species movement is caused by a module array imparting opposing dielectrophoretic forces. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The Clausius-Mossotti factor of the analyte is changed by flushing the analyte with a reference solution, which causes a negative dielectrophoretic force to facilitate release of the analyte. A field effect nanowire or nanoribbon sensor detects the analyte after capture.

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Fredericksburg, VA, United States of America

Monika Weber