Promega Corporation

Monika G Wood

Keith V Wood

Lance P Encell

Mary P Hall

Brock Binkowski

Matthew B Robers

Aldis Darzins

Gediminas Vidugiris

Paul Otto

Dieter Klaubert

Thomas Machleidt

Chad Zimprich

Georgyi V Los

Michael R Slater

Andrew S Dixon

Marie Schwinn

Poncho Meisenheimer

Mark McDougall

James Unch

Thomas A Kirkland

Said A Goueli

Melanie Dart

Nidhi Nath

Hicham Zegzouti

Subhanjan Mondal

Dileep K Pulukkunat

Kris Zimmerman

Kristopher Zimmerman

Rachel Friedman Ohana

Kate Qin Zhao

Frank Fan

Tonny Johnson

Susan Wigdal

Aileen Paguio

Brian Almond

Pete Stecha

Michael P Valley

Brock F Binkowski

Chad Zimbrich

Aldis Darzin

Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.

Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-typeluciferase.

-derived luciferases, novel coelenterazine substrates, and methods of use

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Activation of bioluminescence by structural complementation

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type

Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-typeluciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-typeluciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-typeluciferase.

Syntheticluciferases with enhanced light output

Activation of bioluminescene by structural complementation

Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.

Internal protein tags

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-typeluciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-typeluciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-typeluciferase.

Synthetic oplophorus luciferases with enhanced light output

Growing community of inventors

Mount Horeb, WI, United States of America

Monika G Wood