Uniwersytet Jagiellonski

Instytut Immunologii I Terapii Doswiadczainej Im. Ludwika Hirszfelds Pan

Instytut Immunologii I Terapii Doswiadczalnej Polskiej Akademii Nauk

Instytut Immunologii I Terapii Doswiadczalnej Im. Ludwika Hirszfelda Pan

Monika Brzychczy-Wloch

Andrzej Gamian

Ewa Brzozowska

Sabina Gorska

Tomasz Gosiewski

Sabina Górska

Anna Dobrut

Agata Pietrzyk

Malgorzta Bulanda

Piotr Heczko

The method according to the invention enables the confirmation of infections/carriage caused bybacterial species. The method employs a specific reaction of selected epitopes that are part of immunoreactive proteins of clinical isolates ofwith antibodies present in the serum.

Diagnostic test

An exemplary embodiment describes a method for detection of bacteria and fungi in a sample of biological material, wherein the DNA contained in the sample of biological material is subjected to amplification in multiplex real-time PCR, with the use of primers specific for bacteria in the first stage and primers specific for fungi, and in the second stage, the resulting DNA is amplified using primers and probes differentiating fungi into a group of mold fungi and yeast fungi and bacteria into Gram-positive and Gram-negative bacteria. Another exemplary embodiment refers to oligonucleotide primers for the detection of bacteria and fungi by PCR and a kit for simultaneous detection of bacteria and fungi.

Method for simultaneous detection of bacteria and fungi in a biological preparation by PCR, primers as well as bacteria and fungi detection kit

The method of the invention allows confirmation of infections caused bybacterial species. The method employs a specific reaction of immunoreactive proteins obtained from clinical isolates ofwith antibodies present in the serum of patients.

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Growing community of inventors

Kraków, Poland

Monika Brzychczy-Wloch