Roche Sequencing Solutions, Inc.

Kapa Biosystems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Monica Herrera

Jenny A Johnson

Martin Ranik

Ashley Hayes

Richard Dannebaum

Severine Margeridon

Daleen Badenhorst

Kunchala Rungsrisuriyachai

Smriti Sharma

Yuan Qin

The invention is a novel method of making and using a library such as a sequencing library of single stranded circular nucleic acid templates via splint ligation. In particular, disclosed are methods of making circular target nucleic acid molecules and libraries of such molecules for downstream analysis such as nucleic acid sequencing. The method comprises the steps of adding universal sequences to nucleic acid molecules, rendering single-stranded these nucleic acid molecules with universal sequences on their ends by contacting with a probe complementary to at least a portion of the universal sequences, and allowing the hybridized probe to enable circularization and formation of single-stranded circular (sscDNA) molecules.

Hybridization-extension-ligation strategy for generating circular single-stranded DNA libraries

The invention is a novel method of isolating long nucleic acids from samples suitable for nucleic acid sequencing. The method is especially suitable for isolating low-concentration nucleic acids, e.g., viral nucleic acids, from clinical samples.

Method of isolating nucleic acids for long sequencing reads

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Livermore, CA, United States of America

Monica Herrera