Roussel Uclaf

Calgene Corporation

Aventis Cropsciences Gmbh

Monica Gervais

Danielle Lando

Charles J Van Assche

Jean Michel Bruneau

Toni Alois Voelker

Toni Alols Voelker

Bruno Dumas

Francoise X Jousset

Mireille Jourdan

Max Bergoin

Proteins having the activity of saccharose phosphate synthetase (SPS) and a process for obtaining the same.

Sucrose phosphate synthetase (SPS), a preparation method and cDNA therefor, and use of the cDNA for modifying SPS expression in plant cells

Sucrose phosphate synthase (SPS), a process for its preparation, its DNA, and utilization of DNA encoding SPS to modify the expression of SPS in the plant cells are provided. Methods and compositions for producing SPS protein and DNA encoding SPS from a number of sources are provided. Also provided are transgenes comprising DNA encoding SPS and methods for the production of transgenic plant cells and plants which have extra SPS activity, and thus an increased capacity to synthesize sucrose. Transgenic plant cells and plants with the additional SPS activity exhibit altered carbon partitioning, growth and development, and yield under a variety of conditions.

Modification of sucrose phosphate synthase in plants

A cDNA coating for a protein having saccharose phosphate synthetase activity and a vector permitting the expression of a protein having saccharose phosphate synthetase activity under the control of a promoter directing the said expression.

Saccharose phosphate synthetase (SPS), a preparation method and CDNA

A protein having sucrose phosphate synthetase (SPS) activity is isolated from plants, preferably maize. The protein has a molecular weight of 110-130 dK and contains at least one peptide selected from Thv Trp Ile Lys, Try Val Val Glu Leu Ala Arg, Ser Met Pro Pro Ile Trp Ala Glu Val Met Arg, Leu Arg Pro Asp Gln Asp Try Leu Met His Ile Ser His Arg and Trp Ser His Asp Gly Ala Arg. Isolation is carried out by obtaining an extract from the plant by grinding, centrifugation and filtration; enriching the extract in SPS protein by precipitation in an appropriate solvent such as polyethylene glycol, centrifugation and solubilization of the precipitate obtained in a buffer solution; subjecting the protein thus obtained to low pressure anion exchange chromatography, chromatography on heparin Sepharose and high pressure anion exchange chromatography; and purifying the active fractions obtained by passage through two high pressure chromatography columns. Hybridomas and monoclonal antibodies are prepared from an antigen resulting from high pressure anion exchange chromatography above, antibodies directed specifically against SPS are selected and the antibodies are used to purify the SPS obtained previously. Complementary DNA coding for the SPS is prepared and used to modify expression of the SPS in plant cells.

Sucrose phosphate synthetase isolated from maize

Sucrose phosphate synthase (SPS), its process for preparation, its cDNA, and utilization of cDNA to modify the expression of SPS in the plant cells are provided.

Sucrose phosphate synthase (SPS), its process for preparation its cDNA,

Recombinant plasmids capable of replicating themselves in Escherichia coli containing a complete or partial sequence of the double or single strand DNA or a Densovirus causing a densovirosis in a sensitive insect, a process for obtaining said plasmids and a method of combatting ravager insects.

Growing community of inventors

Saint-Leu-la-Foret, France

Monica Gervais