Qiagen Sciences, LLC

Isoplexis Corporation

Mong Sano Marma

Jerzy Olejnik

Ilia Korboukh

Luisa Andruzzi

Angela DeLucia

Dona Hevroni

Ben McNally

John Andrew Sheridan

Austin Ricker

Timothy Pelletier

Jimmy Adediran

Elizabeth Carpenter

Magnus Besev

The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3'-O position capped by a disulfide-based 3&#x2032;-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3&#x2032;-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

DNA sequencing reaction additive

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3'-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

Methods of using nucleotide analogues

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3'-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

Thiol-containing cleave reagents and oxidative wash

Methods for synthesizing nucleotide analogues with disulfide linkers

Nucleotide analogues

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Natick, MA, United States of America

Mong Sano Marma