Sudzucker Aktiengesellschaft

N.v. Nutricia

Mohammed Munir

Markwart Kunz

Ralf Mattes

Kathrin Klein

Hurbert Schiweck

Hubert Schiweck

Manfred Vogel

The invention relates to sucrose isomerases, to DNA sequences that code for sucrose isomerases, and to novel processes for the production of non-cariogenic sugars.

Preparation of acariogenic sugar substitutes

The invention relates to sucrose isomerases, to DNA sequences coding therefor, and to novel processes for the production of non-cariogenic sugars.

A process for the preparation of long-chain inulin having an average chain length greater than 20 monomer units while simultaneously obtaining glucose and fructose is performed. An aqueous crude inulin suspension with a crude inulin concentration of 20-70% by weight is subjected to enzymatic treatment with inulinase at temperatures of 30.degree.-70.degree. C. During the process the short-chain components are degraded to mono- and disaccharides, and the long-chain inulins are then separated off from the mono- and disaccharides and converted into dry form. The long chain inulins may be separated from the mono- and disaccharides by crystallization by cooling and centrifugation or chromatographically using a strongly acidic cation exchange resin loaded with calcium ions. The long chain inulins can be used as a component in foodstuffs, in conjunction with sweeteners, bulking agents, thickeners, stabilizers, as well as a fat substitute in foodstuffs, a carbohydrate substitute, dietary fiber, as an excipient for pharmacologically active substances, and as an indicator for kidney clearance.

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Kindenheim, Germany

Mohammed Munir