10x Genomics, Inc.

Mohammad Rahimi Lenji

Rajiv Bharadwaj

Tobias Daniel Wheeler

Christopher Hindson

Andrew D Price

Katherine Pfeiffer

Anthony Makarewicz

Bill Kengli Lin

Michael Schnall-Levin

Pranav Patel

Yifeng Yin

Phillip Belgrader

Serge Saxonov

John R Stuelpnagel

Tarjei Sigurd Mikkelsen

Geoffrey McDermott

Zachary Bent

Paul Ryvkin

Zahra Kamila Belhocine

Jessica Michele Terry

Josephine Harada

Elliott Meer

Vijay Kumar Sreenivasa Gopalan

Christopher Joachim O'Keeffe

Michael Ybarra Lucero

Indira Wu

Niranjan Srinivas

Solongo B Ziraldo

Stephane Claude Boutet

Sarah Taylor

Kamila Belhocine

Alireza Salmanzadeh

Yiran Zhang

Martin Sauzade

Lynna Chen

Ivan Akhremichev

Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

Devices, systems, and methods for increasing droplet formation efficiency

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Methods and systems for analysis

Methods and systems for droplet-based single cell barcoding

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for 'tagging' analytes for characterization.

Methods and systems for processing polynucleotides

The invention provides kits, devices, methods, and systems for forming droplets or particles and methods of their use. The devices may be used to form droplets of a size suitable for utilization as microscale chemical reactors, e.g., for genetic sequencing. In general, droplets are formed in a device by flowing a first liquid through a channel and into a droplet formation region including a second liquid, i.e., the continuous phase. The invention allows for more efficient recovery of droplets or processed droplets.

Devices and methods for generating and recovering droplets

Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.

Microfluidic channel networks for partitioning

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

Growing community of inventors

Pleasanton, CA, United States of America

Mohammad Rahimi Lenji