Synthetic Genomics, Inc.

Viridos, Inc.

Moena Aqui

Imad Ajjawi

Eric R Moellering

Leah Soriaga

John Verruto

Andrew Schultz

Nicholas Bauman

Saheed Imam

Kathleen Kwok

Shaun Bailey

Roberto Spreafico

Fedor I Kuzminov

Randor R Radakovits

Jennifer Nominati

Carlos Chavez-Torres

Disclosed herein are mutant photosynthetic microorganisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

High productivity algal mutants having reduced photosynthetic antenna

The invention provides important tools for the use of Chlorophyte organisms, including regulatory sequences useful for the expression of transgenes in such organisms. Also provided are vectors and expression cassettes containing promoters and/or terminators disclosed herein for expression of transgenes in Chlorophyte organisms. Methods of using these tools are also provided.

Regulatory sequences for expression of transgenes

The present invention provides mutant microorganism that have higher lipid productivity than the wild type microorganisms from which they are derived while biomass at levels that are within approximately 50% of wild type biomass productivities under nitrogen replete conditions. Particular mutants produce at least twice as much FAME lipid as wild type while producing at least 75% of the biomass produced by wild type cells under nitrogen replete conditions. Also provided are methods of producing lipid using the mutant strains.

Microorganisms having increased lipid productivity

Disclosed herein are mutant photosynthetic microorgnaisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

The present invention provides mutant microorganism that have higher lipid productivity than the wild type microorganisms from which they are derived while producing biomass at levels that are at least 45% of wild type biomass productivity under nitrogen replete conditions. Particular mutants produce at least 50% as much FAME lipid as wild type while producing at least the amount of biomass produced by wild type cells under nitrogen replete conditions. Also provided are methods of producing lipid using the mutant strains.

Algal mutants with increased lipid productivity

The disclosure generally relates to methods and materials for modulating cell productivity. In particular, the present disclosure provides polynucleotides encoding transcription factor proteins that when overexpressed in microorganisms result in increased in productivity, such as increased biomass productivity. Also disclosed are methods of using the genetically engineered host strains to modulate or increase productivity of host cells such as, for example, algal or heterokont cells. Genetically engineered host cells, such as algal and heterokont cells having increased biomass productivity and bioproducts derived from such host cells are also disclosed.

Compositions and methods for modulating biomass productivity

The disclosure generally relates to methods and materials for modulating cell productivity. In particular, the present disclosure provides polynucleotides and polypeptides that when overexpressed in microorganisms result in increased in productivity, such as increased biomass productivity. Also disclosed are methods of using the polynucleotides and polypeptides to modulate or increase productivity of host cells such as, for example, algal or heterokont cells. Genetically engineered host cells, such as algal and heterokont cells having increased biomass productivity and bioproducts derived from such host cells are also disclosed.

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San Diego, CA, United States of America

Moena Aqui