Mitsui Chemicals, Inc.

Miyuki Tsuruoka

Takeshi Nakamura

Kiyoshi Ito

Toshifumi Yamaki

Teruo Arii

Tadashi Suzuki

Seiya Nikumaru

The invention provides a nitrile hydratase activation protein participating in activation of nitrile hydratase derived from JCM3095, and a gene sequence encoding the same. Further, the invention provides a recombinant plasmid containing the gene, a recombinant plasmid containing the gene and a nitrile hydratase gene, a transformant strain obtained through transformation with the recombinant plasmid, and a process for producing a corresponding amide compound from a nitrile compound using a culture solution and cells obtained by incubating the transformant strain and treated products of the cells.

Protein participating in the activation of nitrile hydratase and gene encoding the same

The present invention provides the amino acid sequence and base sequence of a Pseudonocardia thermophila-derived nitrile hydratase, provides further a method for changing its amino acid sequence and base sequence without substantially changing the functions of said nitrile hydratase, and nitrile hydratases having a base sequence and an amino acid sequence as changed on the basis of said method, and provides furthermore a recombinant plasmid having the gene of said nitrile hydratase, a transformant containing said recombinant plasmid, a method of using said transformant for producing said enzyme, and a method of using said transformant for producing the corresponding amide compound from a nitrile compound.

Nitrile hydratase

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Yachimata, Japan

Miyuki Tsuruoka