Ajinomoto Co., Ltd.

Miyoko Nawata

Hidetsugu Nakazawa

Takane Katayama

Hidehiko Kumagai

Hideyuki Suzuki

Mitsuyoshi Seki

Hideki Okamura

Michinobu Nakamura

A mutant tyrosine repressor that does not require tyrosine to induce expression of tyrosine phenol-lyase gene is obtained by introducing a mutation into a tyrosine repressor. A microorganism which is able to express large amounts of tyrosine phenol-lyase is obtained by introducing the mutant tyrosine repressor into the microorganism. The microorganism is useful for producing L-DOPA.

Mutant tyrosine repressor, a gene encoding the same, and a method for producing L-DOPA

A mutant tyrosine repressor that does not require tyrosine to induce expression of tyrosine phenol-lyase gene is obtained by introducing a mutation into a tyrosine repressor (tyrR), isolated from, or a tyrosine repressor (tyrR) which is encoded by SEQ ID NO. 1 and its derivative. A microorganism which is able to express large amounts of tyrosine phenol-lyase is obtained by introducing the mutant tyrosine repressor into the microorganism. The microorganism is useful for producing L-DOPA.

Mutant tyrosine repressor which does not require tyrosine to induce expression of the tyrosine phenol-lyase gene

The present invention relates to a method which can prevent browning of hydrolyzed protein obtained by enzymatic hydrolysis of vegetable protein material. A vegetable protein material containing saccharides is mixed with the fungal culture and is subjected to enzymatic hydrolysis in a liquid reaction system. The reaction is conducted first at a temperature ranging from 15&#xb0; C. to 39&#xb0; C. with aeration and agitation, and then, after stopping the aeration, the reaction is conducted and completed at a temperature ranging from 40&#xb0; C. to 60&#xb0; C.

Process for producing protein hydrolyzate

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Kawasaki, Japan

Miyoko Nawata