Unitika Ltd.

Mitsubishi Cable Industries, Ltd.

Miwa Watanabe

Tadao Suzuki

Akihiro Shinzaki

Shido Kawase

Keisuke Kurosaka

Ryoko Nakatani

Michie Uchida

Akiko Shimizu

A novel sorbitol dehydrogenase having excellent substrate affinity and substrate specificity which can be used to measure D-sorbitol which occurs in human erythrocytes and serum in a slight amount or D-sorbitol or D-fructose contained in foods, a microorganism for producing such an enzyme, and a process for the production of the enzyme using such a microorganism. Also disclosed is provides a method for the measurement of sorbitol using the foregoing sorbitol dehydrogenase and a reagent for the quantitative determination therefor.

Sorbitol dehydrogenase, microorganism for producing same, process for the production thereof, method for the measurement of sorbitol and reagent for the quantitative determination therefor

A method of enzymatic analysis utilizing a color-development signal amplification system associated with enzymatic cycling of NAD-NADH interconversion in the presence of dehydrogenase and its substrate, wherein the dehydrogenase is selected from the group consisting of alcohol dehydrogenase derived from Zymomonas and amino acid dehydrogenase derived from thermophilic microorganisms is disclosed. The use of alcohol dehydrogenase derived from Zymomonas provides an extremely higher detection sensitivity than that in the conventional method. The use of amino acid dehydrogenase derived from thermophilic microorganisms improves reliability of the method for a longer period of time than that in the conventional method.

Method for enzymatic analysis and reagant therefor

A system or kit for analysis of phosphatase comprising a reagent I containing NADP or NADPH as a main component, and a reagent II containing components for color-development signal amplification utilizing NAD-NADH interconversion, wherein at least one of the hydrogen donors participating in the NAD-NADH interconversion is included in reagent I, but not in reagent II, which eliminates the necessity that the reagent II is further separated into two parts. This enables the preparation of reagent II as a mixture which can be readily used and which can be preserved for a long period of time without 'reagent blank' occurring. Additionally, the present kit eliminates the necessity of mixing reagents I and II immediately before actual use and thus permits periodical analysis of samples with a reagent having the same components for long time periods with reliable and uniform sensitivity.

Analytical system or kit for phosphatase

Growing community of inventors

Kyoto, Japan

Miwa Watanabe