Sekisui Kagaku Kogyo Kabushiki Kaisha

Mituo Nagata

Fumio Kamiyama

Minoru Kohara

Kazutoshi Yamazaki

Yasuhiko Araki

A filler for liquid chromatography, said filler consisting essentially of a hydrophilic ion-exchanger obtained by copolymerizing a mixture of (A) 5 to 90% by weight of a polymerizable monomer containing one polymerizable double bond and at least one carboxyl group per molecule and having a hydrophobic parameter of not more than 2.3, (B) 10 to 95% by weight of a crosslinkable polymerizable monomer containing at least two polymerizable double bonds per molecule and having a hydrophobic parameter of not more than 2.3, and (C) 0 to 85% by weight of a monomer being different from the monomer (A), containing one polymerizable double bond per molecule and having a hydrophobic parameter of not more than 2.3; and a method for separating a biological material such as proteins, enzymes and amino acids by liquid chromatography using the aforesaid filler.

Filler for liquid chromatography useful for separating a hemoglobin

A liquid-chromatographic filler comprising a particulate synthetic polymer, such as glycerol di or tri (meth) acrylate wherein the filler exhibits a behavior such that in chromatography using polyethylene glycol as a sample and water as an eluent, the elution time of the sample increases with a rise in temperature. Separation of water-soluble substances by liquid chromatography can be achieved by using an aqueous medium as an eluent, and this filler.

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Shiga, Japan

Mituo Nagata