Waseda University

Nemoto Kyorindo Co., Ltd.

Tokyo Women's Medical University

Ebm Corporation

Selfusion Inc.

Mitsuo Umezu

Kiyotaka Iwasaki

Young Kwang Park

Takanobu Yagi

Hitoshi Kasegawa

Takashi Tanaka

Teruo Okano

Tatsuya Shimizu

Charles Alfred Vacanti

Koji Kojima

Shigeyuki Ozaki

Hidekazu Sekine

Katsuhisa Sakaguchi

Osamu Endo

Akira Nishikohri

Shunsuke Tsubouchi

Yuji Morimoto

The present invention suppresses the strength reduction or degeneration of a tissue after the tissue is dried and/or sterilized, for the tissue comprising biological components and the like. Specifically, biological tissue is immersed in a trehalose solution and shaken, thereby impregnating the biological tissue with the trehalose solution. The trehalose solution used here is one obtained by dissolving trehalose in a phosphate buffered saline, the concentration of trehalose being preferably in the range of 20 wt % to 35 wt %. Thereafter, the biological tissue is dried to remove moisture in the biological tissue, and sterilized with ethylene oxide gas.

Method for treating biological tissue and biological tissue

To provide a mixing device, a mixing tube, a drug solution injecting system, and a drug solution mixing method capable of evenly and efficiently mixing a plurality of kinds of drug solutions. The mixing device according to the present invention includes: a swirling flow generating chamber; a first inflow opening for introducing a first drug solution into the swirling flow generating chamber in a direction parallel to a central axis of the swirling flow; a second inflow opening for introducing a second drug solution into the swirling flow generating chamber so as to generate a swirling flow of the second drug solution having a specific gravity lower than that of the first drug solution; an outflow opening for discharging a mixed drug solution; and a narrowing chamber which is interposed between the swirling flow generating chamber and the outflow opening and has a space continuously narrowed toward the outflow opening.

Mixing device, mixing tube, drug solution injecting system, and drug solution mixing method

A method for manufacturing a multilayered cell sheet characterized in fabricating a vascular bed that constructs a vascular network extending to the surface from a channel for perfusing a medium, the channel being embedded in a gel; and layering a cell sheet onto the vascular bed to construct a vascular network in the cell sheet. This manufacturing method makes it possible to construct a vascular network in the cell sheet and to fabricate a thick multilayered cell sheet in a simple manner by layering cell sheets. Such a thick multilayered cell sheet is useful as an in-vivo tissue substitute in regenerative medicine involving a variety of tissues.

Method for manufacturing multilayered cell sheet, multilayered cell sheet having vascular network obtained thereby, and method of use thereof

An artificial mitral valve has a ring, an anterior cusp-side region, a posterior cusp-side region and a valve leaflet connected along an outer edge of the ring. The valve leaflet includes an anterior cusp forming member connected to the anterior cusp-side region and a posterior cusp forming member connected to the posterior cusp-side region. The anterior cusp forming member is provided with has an upper edge joined to the ring and a lower edge that forms a bifurcated portion. The upper edge is made up of a pair of right and left edges that incline inward and upward from the right and left sides, and a curved edge interposed between the inclined edges that curves along the anterior cusp-side region. The posterior cusp forming member has an upper edge joined to the ring and a lower edge that forms a bifurcated portion.

Artificial valve leaflet

A system () for forming and maintaining a biological tissue by which a biological tissue can be artificially formed by culturing cells, which comprises a pulse pump (), a circulation pathway () having such a circuit structure as allowing a liquid cell culture medium discharged from the pulse pump () to return into the pulse pump (), and a cell culture section (A) and a gas exchange section (B) provided along the circulation pathway (). The cell culture section (A) holds a cell holder (H) in such a manner to form a first channel wherein the liquid cell culture medium flowing in the circulation pathway () passes through the cell holder (H) and returns into the circulation pathway () and a second channel wherein the liquid cell culture medium flowing in the circulation pathway () passes outside the cell holder (H) and returns into the circulation pathway thereby bringing about a difference in pressure between the liquid cell culture medium passing through the respective channels.

System for forming and maintaining biological tissue

Disclosed is a method whereby a narrowed part similar to an actual lesioned blood vessel can be easily obtained. A mold () comprises first and second molded members () having inner spaces () passing through in the axial direction and an axial member () detachably inserted through the individual inner spaces () of the individual molded members (). The molded members () have tapered front parts () respectively. In the state of facing the front ends () of the tapered front parts () to each other and thus forming a concave (), the axial member () is attached. A first material () made of a mixed material comprising calcium carbonate or the like and silicone or the like is applied to the concave (). A second material () such as silicone is applied to the entire outer periphery of the mold (). After hardening the materials (), the mold () is withdrawn by detaching the axial member () and dividing the concave (). Thus, a simulated blood vessel () having a simulated narrowed part of the blood vessel caused by calcification can be obtained.

Mold for producing simulated blood vessel, method of producing simulated blood vessel and simulated blood vessel

An artificial mitral valve has a ring, an anterior cusp-side region, a posterior cusp-side region and a valve leaflet connected along an outer edge of the ring. The valve leaflet includes an anterior cusp forming member connected to the anterior cusp-side region and a posterior cusp forming member connected to the posterior cusp-side region. The anterior cusp forming member has an upper edge joined to the ring and a lower edge that forms a bifurcated portion. The upper edge is made up of a pair of right and left edges that incline inward and upward from the right and left sides, and a curved edge interposed between the inclined edges that curves along the anterior cusp-side region. The posterior cusp forming member has an upper edge joined to the ring and a lower edge that forms a bifurcated portion.

Stentless artificial mitral valve

It is intended to provide an endurance test apparatus whereby the behaviors of a vessel caused by a biomechanical burden can be simulated using a relatively simple constitution so that the endurance of a medical instrument (for example, a stent or an artificial vessel after anastomosis) can be tested in a similar state to an actual vessel, and an endurance test method therefor. Namely, an endurance test apparatus () comprises a simulated vessel () made of an elastic body, first and second holding members () respectively supporting the both ends in the axial direction of the simulated vessel () and making the both ends relatively rotatable and interval-adjustable, and first and second motors () operating these holding members (). The simulated vessel () is supported by the first and second holding members () in a state stretched from its inherent length under a tensile force. Due to the driving motors (), the holding members () elongate and contract the simulated vessel (), which is in the stretched state as described above, while twisting it.

Endurance test apparatus for medical instrument and endurance test method

A blood vessel model for medical training not deforming with time and approximated to a real blood vessel without using any other tool when the blood vessel wall of the simulated blood vessel is incised or cut off. The blood vessel modelincludes a simulated blood vesselsimulating a blood vessel and artificially fabricated and a basesupporting the simulated blood vessel. The simulated blood vesselhas a circumferentially exposed part and the other part buried in the base. The basehas a jointed partdisposed in a position below the simulated blood vesseland formed by joining forming edgesA,A of a cutoutextending along the simulated blood vesselto impart a tensile stress in the diameter-increasing direction to the simulated blood vessel

Blood vessel model for medical training and method for manufacturing same

In the transplant of a living organism tissue, such as a heart valve, taken from an animal, etc. into a human body, a cell removing solution for removing original cells from the living organism tissue is provided with flow approximately equal to the bloodstream of transplant recipient living body, and the living organism tissue is placed in the flow so as to effect immersion of the living organism tissue in the cell removing solution. In the immersion, it is preferred that the living organism tissue placed in the cell removing solution, while being rotated, be irradiated with microwave. As a result, original cells can be removed from the living organism tissue uniformly and reliably, so that the biocompatibility of living organism tissue after transplant can be enhanced.

Growing community of inventors

Tokyo, Japan

Mitsuo Umezu