Hitachi, Ltd.

Mitsuo Shimada

Yoshio Fujii

Sadabumi Ohnuma

A detecting device is disposed between a separation column to which an eluent is supplied and a flow passage resisting member is located in a flow passage leading to a liquid discharge exit. This detecting device includes a sample cell and a reference cell. The effluent from the separation column is introduced into the flow passage resisting member through the sample cell. A change-over valve is disposed in the flow passage between the separation column and the sample cell and also is connected to one end of the reference cell. The other end of the reference cell is connected to the flow passage between the sample cell and the flow passage resisting member, whereby the reference cell and sample cell are kept communicated with each other. After an eluent has been filled in the reference cell, the communication of the flow passage between the separation column and the sample cell with the reference cell is intercepted by the change-over valve. While this interception is maintained, an effluent from the separation column is passed through the sample cell, and concentrations of respective components in the sample solution are measured by comparing the sample solution with the reference solution in the reference cell with respect to the refractive index or absorbance. Since the noise and drift can be reduced according to this system, the measurement accuracy can be highly improved.

Liquid chromatograph apparatus

A high sensitivity absorptiometer comprises a light source lamp accompanied inevitably with heat generation, a sample container through which the light from the light source is transmitted and a detector device for detecting the light which has transmitted through the sample container. The light emitted from the light source lamp and directed to the sample container is adapted to pass through a chamber which is formed fluid-tight on a surface portion of the light source lamp or through a solid state optical conductor such as optical fiber having a light inlet end face closely fitted to the surface portion of the lamp, thereby to suppress movement and turbulence of air as caused by heat generated by the light source lamp with a view to excluding noise and drift ascribable to the air movement or turbulence phenomenon from the signal obtained from the detector device.

Growing community of inventors

Katsuta, Japan

Mitsuo Shimada