Ngk Insulators, Inc.

Other

Biseiken Co., Ltd.

Mitsuo Kawase

Yasuko Yoshida

Kazunari Yamada

Tadahiko Inukai

Takaaki Hasegawa

Yasuo Suzuki

Toshikazu Hirota

Shin Yamamoto

Shinji Iijima

Kousuke Niwa

Katsuhide Miyake

Naoki Murata

Hitoshi Yonekawa

Michio Ohta

Isao Miyazaki

Chiho Matsuda

Masayuki Nadai

Yuji Kawase

Kenichi Miyamoto

Tadashi Nomura

Kazukiyo Kobayashi

An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer comprising an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence, and a second primer having a label are prepared. The first primer and the second primer are used for the target nucleic acid in a sample to amplify a chimeric DNA having a tag sequence and the label. The chimeric DNA is hybridized with a detection probe on a solid phase to obtain signal intensity information based on the label, and the target nucleic acid is detected based on the signal intensity information.

Method for detection of target nucleic acid

A method for hybridizing nucleic acids, which includes an annealing step of preparing a first single stranded nucleic acid fragment immobilized on a surface of an immobilizing material and a second single stranded nucleic acid fragment labeled with fluorescence or radioisotope and forming a complementary double strand from the first single stranded nucleic acid fragment and the second single stranded nucleic acid fragment, and an enzyme treatment step. In the annealing step, the complementary double strand is formed by performing a temperature gradient processing performed from a high temperature area to a low temperature area, and in the enzyme treatment step, a noncomplementary nucleic acid portion contained in the complementary double stand is recognized and cleaved by adding endonuclease. This method has high measurement sensitivity and the operation is simple.

Method for highly sensitive hybridization of nucleic acids, and method for gene analysis using the same

Therapeutic drugs for endotoxin blood symptom and multi-organ failure induced by it is provided, which therapeutic during are composed of sialic acid its salt, polymers of sialic acid or a salt of the polymer as effective components and have high therapeutic effects for shock death and organ failure induced by endotoxin as well as high safety, and are effective for treatment of endotoxin-shock, and organ failure.

Therapeutic drug for endotoxin blood symptom and multi-organ failure induced thereby

A virus-removing filter using, as a virus-capturing body, at least one kind of,type II culture and Type II polysaccharide.

Virus-removing filter

Method of inhibiting cancer metastasis

Cancer metastasis inhibitor containing a streptococcus agalactiae Ia

A virus-removing filter using, as a virus-capturing body, at least one kind of sialic acid, a sialic acid derivative, and sugars, glycoproteins and glycolipids containing sialic acid and/or the sialic acid derivative.

An enzyme-fixed bioreactor, including a reaction column, enzyme-fixed catalyst particles uniformly and densely filled in the reaction column, the catalyst particles being composed of carrier sepiolite particles consisting essentially of sepiolite and an enzyme carried on the surface of the carrier sepiolite particles. The bioreactor has stable heat resistant and chemical properties, high productivity, and is economically superior to prior art, without fear of destruction of the catalyst, short path, and clogging in the reaction column.

Enzyme-fixed bioreactor

Proliferation activity of methane bacteria in organic waste water can be improved by maintaining the ratio of the amounts of nickel, iron and cobalt contained in the waste water to the BOD thereof to not lower than certain values, and various organic waste waters, such as waste liquor formed during the production of beer and the like, can be treated in a high BOD removal ratio of not lower than 90% while maintaining a high BOD load of not lower than 10 kg/m.sup.3 .multidot.day.

Method for treating organic waste by methane fermentation

Growing community of inventors

Chita, Japan

Mitsuo Kawase