Fujirebio Inc.

Nippon Paint Compnay, Ltd.

Fujirebio Inc. (Fujirebio Kabushiki Kaisha)

Mitsuo Isomura

Yoshihiro Ashihara

Akira Hasegawa

Masahiko Matsukawa

Masahisa Okada

Motohiro Sasaki

Katsuaki Yoshioka

Hiroko Saruta

Atsuka Sato

Makoto Anan

Yuko Ishioka

Disclosed are an immunoassay device which comprises a labeled substance dotting portion and a specimen dotting portion provided thereon, and an immunoassay method using the device.

Immunoassay device and immunoassay method using the same

An enzyme-labeled immunoassay is performed by the steps of allowing a test sample to react with an enzyme-labeled reagent, allowing a substrate to react with the enzyme to form a signal, and immobilising the enzyme-labeled reagent, with the prevention of a further signal formation from a predetermined time on after the immobilisation of the enzyme-labeled reagent, using an enzyme inhibitor. A device for performing this enzyme-labeled immunoassay includes an absorbent material capable of transporting a developing solution by capillary action, the absorbent material including (a) a developing liquid application zone, (b) an enzyme-labeled reagent zone containing an enzyme-labeled reagent, (c) a sample receiving zone, and (d) an indicator reagent zone capable of immobilising the enzyme-labeled reagent after the reaction of the test sample with the enzyme-labeled reagent in an amount dependent on the assay result, with an enzyme inhibitor being applied to a portion in the absorbent material upstream of the enzyme-labeled reagent zone, which enzyme inhibitor prevents the formation of a signal from a predetermined time on after the enzyme-labeled reagent is immobilised at the indicator reagent zone.

Enzyme-labeled immunoassay and device therefor

There are disclosed a magnetic particle for an immunoassay method, which comprises a core and a coating layer formed on the surface of the core wherein said core comprises an organic polymer matrix and said coating layer comprises a mixed crystal ferrite represented by the formula:

Magnetic particle and immunoassay using the same

Growing community of inventors

Tokyo, Japan

Mitsuo Isomura