Takara Shuzo Co., Inc.

Takara Bio Inc.

Mitsuhiro Ueno

Ikunoshin Kato

Kazutoh Takesako

Naoyuki Awazu

Kiyozo Asada

Kei Fujinaga

Takashi Ueno

Hideto Chono

Kazuo Shimanaka

Kimikazu Hashino

Mutsumi Sano

Mio Morishita

Hirofumi Yoshioka

Keiji Tanaka

Yoshimi Onishi

Tsuyoshi Miyamura

Hideharu Saito

Hajime Matsumura

Tomoko Iwasaki

Haruko Konishi

Yoko Uno

A method of transferring a foreign gene into cells, characterized by involving: the step of transferring into the cells with the use of an adenovirus vector, a first nucleic acid, which has a sequence provided with adeno-associated virus-origin ITRs in both sides of the target foreign gene to be transferred, and a second nucleic acid, which has an adeno-associated virus-origin rep gene and a promoter for expressing this gene and carries a stuffer sequence inserted thereinto sandwiched in two recombinase recognition sequences and located between the rep gene and the promoter; and the step of expressing the Rep protein under the action of recombinase in the cells obtained in the above step to thereby integrate the target foreign gene into the chromosomal DNA.

Gene transfer method

Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferrin receptor, or introducing a new functional group into the functional substance.

Gene transfer methods

Antibiotic TKR459, production method, and microorganism

Antibiotic TKR2999, process for the preparation thereof and microbe

The present invention has for its object to provide novel biologically active substances which is of value as a therapeutic agent for fungal infections and immune disorders. This invention is related to a biologically active substance TKR2449 analog(s) which is represented by the following general formula (A);

Physiologically active substances TKR2449, process for producing the same, and microorganism

Antibitic TKR2648 having the following chemical formula (I) or its pharmacologically acceptable salt.

Antibiotic TKR2648 and process for producing the same

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Kusatsu, Japan

Mitsuhiro Ueno