Daiichi Pure Chemicals Co., Ltd.

Toshiba Silicone Co., Ltd.

Mitsuhiro Nakamura

Mitsuhisa Manabe

Koichi Hino

Mitsuaki Yamamoto

Kazuo Nakanishi

Yuriko Taniguchi

Kazunori Saito

Shoko Yamamoto

Nobuyuki Kawai

A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.

Method of pretreatment of sample for quantitating cholesterol and method for quantitating cholesterol in specific lipoproteins by using the same

A method of lipid assay characterized by assaying the lipids contained in a blood component in the presence of an organic silicon compound. The method can cause specific conditions for direct methods while satisfying requirements such as no influence on precision of assay, no burden on assay apparatus, and easy availability.

Method of lipid assay and reagent for use therein

A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.

Method for quantitatively determining LDL cholesterols

Method for quantitatively determining cholesterol

A push button switch which may be used, for example, as a zooming switch in a video camera, having a uniform electrical resistance/applied pressure characteristic and which can be manufactured easily and at a low cost. The switch includes a conductive layer and a pressure-sensitive resistance element disposed between electrode patterns provided on a printed circuit board and an insulated push button. The conductive layer is coated with a pressure-sensitive conductive paste and the pressure-sensitive resistance element is fixed to the insulated push button at a opposed surface to the electrode patterns separated by a predetermined distance.

Push button switch

Growing community of inventors

Ryugasaki, Japan

Mitsuhiro Nakamura