Cardea Bio, Inc.

USA As Represented by Secretary of the Navy

Nanomedical Diagnostics, Inc.

Other

Edico Genome Corporation

Agilome, Inc.

Paragraf USA Inc.

Mitchell Lerner

Paul Robert Hoffman

Brett R Goldsmith

Pieter Van Rooyen

Deng Pan

John D Rockway

Oren Sternberg

Israel Perez

Savannah J Afsahi

Nicholas A Lumsden

An apparatus may include a sensor chip fabricated on a semiconductor wafer, the sensor chip may include a graphene channel patterned in a graphene layer disposed on a dielectric substrate. The sensor chip may include a first electrode formed in electrode material so as to form one or more of an edge side contact or a top side contact in electrical contact with a first end of the graphene channel. The sensor chip may include a second electrode formed in electrode material so as to form one or more of an edge side contact or a top-side contact in electrical contact with a second end of the graphene channel. The sensor chip may include an insulation layer that is layered above at least of portion of the graphene layer and is selected from an inorganic oxide layer and an organic layer.

Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same

An apparatus includes a biosensor integrated circuit (IC) chip with sensing zones and/or well structures configured to receive a liquid with biological analytes. The chip includes passivation and etch stop layers with an opening over a channel layer and an array of liquid gated field effect transistors with a 2D channel disposed on a dielectric oxide layer. A conductive drain and a conductive source form edge and/or top side contacts with opposite ends of the 2D channel. The chip further includes reference electrodes formed in a metal layer, configured to contact the liquid, and disposed at a horizontal distance apart from the graphene channels. The transistors are operable to enable a set of measurements to sense parameters of the biological analytes based on changes in a shape of Id-Vgs transconductance curves. A system and a method have similar structures and perform the functions of the apparatus.

An integrated circuit (IC) chip includes ROIC circuitry in a CMOS wafer with a top dielectric layer and at least one graphene field effect transistor (gFET) sensor array added above the CMOS wafer. The IC chip includes access transistors controlled by the ROIC circuitry and further includes sensing circuitry which includes the at least one gFET sensor array and a passivation opening that allows direct contact of a sample liquid with the graphene channels of the gFETs in the at least one gFET sensor array, such that a liquid gate is formed above the graphene channel upon receipt of the sample liquid. In some examples, the IC chip includes a process, memory controller, and memory. A system and a method have similar structures and perform the functions of the apparatus.

Ic with graphene fet sensor array patterned in layers above circuitry formed in a silicon based cmos wafer

An apparatus includes a biosensor integrated circuit (IC) chip having multiple well structures configured to receive a liquid comprising one or more biological analytes. The well structures include a passivation layer with an opening over one or more field effect transistors (gFETs) which include a layer of 2D channel material selected from molybdenum disulfide (MoS) and graphene; a drain electrode connected to a first end of the channel; a source electrode connected to a second end of the channel, wherein the individual gFETs are configured such that liquid received by the well structure is confined to form a liquid gate above a top surface of the channel. A system and method perform various functions of the apparatus.

Two-dimensional channel FET devices, systems, and methods of using the same for sequencing nucleic acids

An apparatus includes a biosensor integrated circuit (IC) chip with sensing zones and/or well structures configured to receive a liquid with biological analytes. The chip includes a passivation layer with an opening over a channel layer and an array of graphene field effect transistors (gFETs) individually having a 2D graphene channel disposed on a dielectric oxide layer, a conductive drain, and a conductive source. A liquid gate is formed above the top surface of the graphene channel. The chip further includes reference electrodes formed in a metal layer, configured to contact the liquid, and disposed at a horizontal distance apart from the graphene channels. The individual gFETs are operable to enable a set of measurements to sense parameters of the biological analytes based on changes in a shape of Id-Vgs transconductance curves. A system and a method have similar structures and perform the functions of the apparatus.

This invention concerns Chemically-sensitive Field Effect Transistors (ChemFETs) that are preferably fabricated using semiconductor fabrication methods on a semiconductor wafer, and in preferred embodiments, on top of an integrated circuit structure made using semiconductor fabrication methods. The instant ChemFETs typically comprise a conductive source, a conductive drain, and a channel composed of a one-dimensional (1D) or two-dimensional (2D) transistor nanomaterial, which channel extends from the source to the drain and is fabricated using semiconductor fabrication techniques on top of a wafer. The ChemFET also includes a gate, often the gate voltage is provided through a fluid or solution proximate the ChemFET. Such ChemFETs, preferably configured in independently addressable arrays, may be employed to detect a presence and/or concentration changes of various analyte types in chemical and/or biological samples, including nucleic acid hybridization and/or sequencing reactions.

Chemically-sensitive field effect transistors for biosensor chips and system are disclosed. The itransisitors have a multi-layered structure for performing a set of measurements of a biological reaction involving a binding event for one or more biological analytes that may be label-free. The multilayer structure includes a first insulating layer above a substrate layer and a source electrode and a drain electrode disposed positioned over the first insulating layer; a second insulating layer above the first insulating layer and proximate the source and drain electrodes forming side wall members of a well for a fluid comprising the analytes; a 2D graphene layer forming a channel between source and drain electrodes; a solution gate, formed by fluid flowed over the channel, configured to enable determining differences between one or more sample I-Vg curves having a shifted and changed shape relative to a reference curve; embodiments may include ion-selective membranes and/or ion getters.

Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids

Provided herein are devices, systems, and methods of employing the same for the performance of bioinformatics analysis. The apparatuses and methods of the disclosure are directed in part to large scale graphene FET sensors, arrays, and integrated circuits employing the same for analyte measurements. The present GFET sensors, arrays, and integrated circuits may be fabricated using conventional CMOS processing techniques based on improved GFET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense GFET sensor based arrays. Improved fabrication techniques employing graphene as a reaction layer provide for rapid data acquisition from small sensors to large and dense arrays of sensors. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes, including DNA hybridization and/or sequencing reactions. Accordingly, GFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis within a gated reaction chamber of the GFET based sensor.

The present disclosure is directed towards systems and methods for transferring graphene from the surface of one substrate to another. In one particular embodiment, the graphene layer is grown on a surface of a first substrate, where the bottom of the first substrate is then affixed to the surface of a second substrate. The second substrate may include material made of a rigid or semi-rigid composition to provide structural support and backing to the first substrate. The graphene layer may then be delaminated from the first substrate and transferred to a target surface, such as the surface of an electronic device or biosensor.

Systems and methods for transferring graphene

The present disclosure is directed towards systems for transferring graphene from the surface of one substrate to another. In one particular embodiment, the graphene layer is grown on a surface of a first substrate, where the bottom of the first substrate is then affixed to the surface of a second substrate. The second substrate may include material made of a rigid or semi-rigid composition to provide structural support and backing to the first substrate. The graphene layer may then be delaminated from the first substrate and transferred to a target surface, such as the surface of an electronic device or biosensor.

Systems for transferring graphene

A system includes a very low frequency (VLF) antenna connected to a circuit that has a high pass filter and a low pass filter. The circuit is configured for noise reduction and frequency localization of detected VLF signals. A receiver is configured to receive filtered VLF signals output by the circuit and to separate desired signals for processing. A display is configured to provide a user with visual feedback based at least in part on an even signature extracted by a digital signal processor (DSP).

System and method for very low frequency detection of an event signature

Methods for fabricating a graphene nanoribbon array in accordance with several embodiments of the present invention can include the steps of depositing PMMA dots on a substrate in an m&#xd7;n grid, to selectively seed graphene flakes on the substrate by controlling the growth of the graphene flakes on the substrate during the graphene deposition. The methods can further include the steps of masking the graphene flake edges with an insulator layer, at a very low deposition time or at a lower precursor concentration, to ensure there are not enough insulator molecules to form a complete layer over the flakes, but only enough insulator to form around the flakes edges. Once the graphene flake edges are masked, the bulk graphene can be etched, and the masking insulator can be removed to expose the resulting graphene nanoribbon.

Scalable graphene nanoribbon arrays for digital transistors

A chemically-sensitive field effect transistor is disclosed herein. The chemically-sensitive field effect transistor comprises a CMOS structure comprising a conductive source and a conductive drain, a channel and an analyte-sensitive dielectric layer. The channel extends from the conductive source to the conductive drain. The channel is composed of a one-dimensional transistor material or a two-dimensional transistor material. The analyte-sensitive dielectric layer is disposed over the channel. An I-V curve or an I-Vcurve is shifted in response to a chemical reaction occurring on or near the chemically-sensitive field effect transistor.

Chemically-sensitive field effect transistor

A transparent antenna is fabricated by combining a metal nanomesh structure and a graphene sheet. The nanomesh structure is formed on a surface, and the graphene sheet is placed over the nanomesh structure. The graphene sheet is adhered to the nanomesh structure to form a graphene nanomesh structure. The graphene nanomesh structure is shaped to form the transparent antenna that efficiently transmits and receives signals in a desired frequency range yet is optically transparent.

Growing community of inventors

San Diego, CA, United States of America

Mitchell Lerner