Mitsubishi Kasei Corporation

Mishio Kawamura

Takao Uchiyama

Haruyuki Ohkishi

Sachiko Ohba

Makoto Ueda

Reiko Sashida

Kuniji Tanaka

The present invention is directed to a method of manufacturing inulotriose and/or inulotetrose by using an exo-type hydrolase capable of hydrolyzing a fructan only every 3 or 4 sugar units from a terminal fructose thereof, so as to produce inulotriose and/or inulotetrose, and of recovering the inulotriose and/or inulotetrose. The enzyme is produced by Streptomyces sp. MCI 2423 (FERM BP-2678) and Aureobacterium sp. MCI 2494 (FERM BP-2679).

Method of manufacturing inulotriose and/or inulotetrose using an

The present invention is directed to an exo-type hydrolase capable of hydrolyzing a fructan only every 3 or 4 sugar units from a terminal fructose thereof to produce predominantly inulotriose and/or inulotetrose. The enzyme is produced by Streptomyces sp. MCI 2423 (FERM BP-2678) and Aureobacterium sp. MCI 2494 (FERM BP-2679).

Exo-type hydrolase capable of hydrolyzing a fructan only every 3 or 4

Disclosed herein is a process for preparing difructose dianhydride III (DFA III) comprising reacting inulin with an inulin lytic enzyme derived from a microorganism belonging to Arthrobacter ilicis. The enzyme employed herein efficiently produces DFA III from inulin and is more stable against heat than conventional enzymes. The present process enables industrial continuous production of DFA III. The preferred strain used herein is Arthrobacter ilicis MCI 2297 (FERM P-9893).

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Toyonaka, Japan

Mishio Kawamura