The Brigham and Women's Hospital, Inc.

The Broad Institute, Inc.

Miseon Lee

Amit Choudhary

Basudeb Maji

Bridget K Wagner

Soumyashree Ashok Gangopadhyay

Debasish Manna

Kisuk Yang

Mengchao Shi

Peter Jones

3D cell cultures and devices for 3D cell culture, and methods of use thereof are provided. In some embodiments, the 3D cell culture comprise pancreatic β cells and can be generated in multi-well plates, allowing for high throughput assays on the cell culture.

Three-dimensional cell culture, devices, and use thereof

Compositions and methods for inhibiting the activity of RNA-guided endonucleases, and methods for identifying such compositions are included. Methods of treatment using the inhibitory compounds, formulations and methods are also detailed, as well as control of nucleic acid editing systems and methods using said compounds.

Inhibitors of RNA-guided nuclease target binding and uses thereof

The disclosure includes zinc prodrugs for targeted delivery of therapeutic, diagnostic or imaging agents to β-cells and methods of use therefor. The disclosure also includes targeted delivery of small molecules to β-cells that stabilize and activate CRISPR effector proteins comprising at least one destabilization domain, to enable CRISPR-based genome editing and transcriptional activation or repression in β-cells.

Targeted delivery to beta cells

Growing community of inventors

Cambridge, MA, United States of America

Miseon Lee