Suntory Holdings Limited

Misa Ochiai

Eiichiro Ono

Harukazu Fukami

Masahiro Nakao

Jun Ogawa

Eiji Sakuradani

Kazunari Iwaki

Tadayoshi Hirai

Hiroaki Kasai

Hisanori Tokuda

Hiroshi Kawashima

Sakayu Shimizu

Toshihiko Ashikari

Akio Noguchi

Akinori Ando

Masaki Kanamori

Katsuro Miyagawa

Koji Nagao

Soichiro Urai

Takehiro Watanabe

Kohki Fujikawa

Maki Asami

The purpose of the present invention is to: determine the structure of a novel steviol glycoside which is detected from species containing a large amount of Reb. C (also referred to as dulcoside B), and a trace amount of which is capable of influencing the quality of taste; and understand the taste characteristics of said steviol glycoside. The present invention provides a compound represented by formula (1) or a salt thereof, or a hydrate thereof.

Steviol glycoside and production method therefor, and sweetener composition containing same

The purpose of the present invention is to provide a steviol glycoside hexose transferase, and a method for producing a steviol glycoside that contains glucose and/or rhamnose using said enzyme. The present invention provides a steviol glycoside hexose transferase, and a method for producing a steviol glycoside that contains glucose and/or rhamnose using said enzyme. The present invention also provides a transformant into which a steviol glycoside hexose transferase gene has been introduced, and a method for preparing said transformant.

Steviol glycoside hexose transferase and gene coding for same

The purpose of the present invention is to provide a protein, said protein having an activity of synthesizing rhamnose from glucose, and a polynucleotide encoding the same. Provided are a rhamnose synthase derived from stevia and a method for producing rhamnose from glucose using a gene. Also provided is a method for producing a steviol glycoside using the rhamnose synthase derived from stevia.

Rhamnose synthase derived from stevia and gene

The present invention provides a method for producing a steviol glycoside and/or steviol, said method including a step in which a steviol glycoside having at least one unbranched &#x3b2;1,2-glycosidic bond is reacted with the glycosidase AOBGL1 and/or AOBGL3, or a variant thereof, so as to cleave the &#x3b2;1,2-glycosidic bond.

Method for producing steviol and steviol glycoside using AOBGL1 homolog

A novel method for producing a mogrol glycoside and mogrol has been required. The present invention provides a method for producing a mogrol glycoside and/or mogrol, said method comprising a step for cleaving at least one glucoside bond in a mogrol glycoside.

Method for producing mogrol or mogrol glycoside

There has been a demand for a new method for producing a steviol glycoside and steviol. The present invention provides a method for producing a steviol glycoside and/or steviol, the method comprising a step of breaking bonds of steviol glycoside: a glucosidic bond at position 13; a glucosyl ester bond at position 19; and/or a glycosidic bond in a side chain (excluding a rhamnoside bond).

Method for producing steviol and steviol glycoside by using AOBGL11 homolog

To provide a mogrol glycosyltransferase and a method for producing a mogrol glycoside using the enzyme. The present invention provides a mogrol glycosyltransferase and a method for producing a mogrol glycoside using the enzyme, and a transformant into which a mogrol glycosyltransferase gene is introduced and a method for preparing the transformant.

Mogrol glycosyltransferase and gene encoding same

The present invention provides a method for preparing a mogroside having no &#x3b2;-1,6-glucoside bond comprising the step of reacting glycosidase ASBGL2, AOBGL2, AOBGL1, ASBGL1, or a variant thereof with a mogroside having at least one &#x3b2;-1,6-glucoside bond, thereby cleaving said &#x3b2;-1,6-glucoside bond.

Method for preparing mogroside having no &#x3b2;-1,6-glucoside bond

The present invention provides a method for preparing a steviol glycoside and/or steviol comprising reacting glycosidase AOBGL1 and/or AOBGL3 or a variant thereof with a steviol glycoside having at least one monoglucoside bond and/or monoglucosyl ester bond, thereby cleaving said monoglucoside bond and/or monoglucosyl ester bond.

Method for producing steviol and steviol glycoside using AOBGL3 homolog

The present invention aims to provide a promoter showing high expression activity in microorganisms belonging to the genus. The present invention provides a polynucleotide which contains any one nucleotide sequence selected from the group consisting of SEQ ID NO: 1 to 28, or a variant thereof.

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The present invention aims to provide a promoter showing high expression activity in microorganisms belonging to the genus. The present invention provides a polynucleotide which contains any one nucleotide sequence selected from the group consisting of SEQ ID NOs: 1 to 28, or a variant thereof.

Promoter exhibiting high expression activity in mortierella microorganisms

Steviol glucosyltransferases and methods for producing steviol glycosides using the enzymes are provided. The present invention provides steviol glucosyltransferases and methods for producing steviol glycosides using the enzymes. The invention also provides transformants into which steviol glucosyltransferase genes are introduced and methods for preparing the transformants.

Steviol glucosyltransferases and genes encoding the same

The present invention relates to an acyl-CoA synthetase homolog protein from microorganisms of the genus, a polynucleotide encoding the protein, and so on. The invention provides polynucleotides comprising an acyl-CoA synthetase homolog protein gene from, e.g.,, expression vectors comprising these polynucleotides and transformants thereof, a method for producing lipids or fatty acids using the transformants, food products containing the lipids or fatty acids produced by the method, etc.

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Osaka, Japan

Misa Ochiai